Project description:Cells were transfected with plasmids containing AsCas12f variants and sgRNAs for gene-editing. Off-target effect was determined using GUIDE-seq on an Illumina Nextseq platform.
Project description:Use DNase-seq to assess genome-wide chromation remodeling which occurred in CRISPR/Cas9 and TALE genome engineering systems Determining chromatin structural changes in transfected cells vs. the parent HEK293T cells
Project description:Interventions: a.The compression gloves and stockings were developed by a team from the biomedical engineering program, faculty of engineering, Chulalongkorn University.
b.Pressure adjustable in range of 20-33 hPa of pressure in the experimental group and no pressure in the control group.
c.This pressure level is demonstrated as a safety profile in previous literature.
,No treatment;Experimental Device,No Intervention No treatment;The compression gloves and stockings,Control
Primary outcome(s): incidence of grade 2 or higher OIPN according to NCI-CTCAE 6 months NCI-CTCAE
Study Design: Randomized