Project description:Cells were transfected with plasmids containing AsCas12f variants and sgRNAs for gene-editing. Off-target effect was determined using GUIDE-seq on an Illumina Nextseq platform.
2023-04-24 | GSE211600 | GEO
Project description:AsCas12f-mediated editing
| PRJNA668665 | ENA
Project description:AsCas12f-related HTS data
| PRJNA668664 | ENA
Project description:Gene-editing effciency of engineered AsCas12f variants
Project description:Use DNase-seq to assess genome-wide chromation remodeling which occurred in CRISPR/Cas9 and TALE genome engineering systems Determining chromatin structural changes in transfected cells vs. the parent HEK293T cells
Project description:Interventions: a.The compression gloves and stockings were developed by a team from the biomedical engineering program, faculty of engineering, Chulalongkorn University.
b.Pressure adjustable in range of 20-33 hPa of pressure in the experimental group and no pressure in the control group.
c.This pressure level is demonstrated as a safety profile in previous literature.
,No treatment;Experimental Device,No Intervention No treatment;The compression gloves and stockings,Control
Primary outcome(s): incidence of grade 2 or higher OIPN according to NCI-CTCAE 6 months NCI-CTCAE
Study Design: Randomized
Project description:We developed a multi recombinase engineering rationale, that combines oligonucleotide recombineering with the selective capacity of antibiotic resistance via transient insertion of selector plasmids. We tested this method in Mycoplasma pneumoniae, a bacterium with a very inefficient native recombination machinery. A wide variety of targeted genome modifications were carried out. We did whole genome sequencing of some clones to confirm that the engineering method is not mutagenic and ensure that genome modifications only occurred at the intended loci. Specifically we sequenced clones carrying 1 kb deletion at 4 different chromosomal locations (i.e., M129-GP35-PtetCre Δ1kbmpn088::lox scar, M129-GP35-PtetCre Δ1kbmpn256::lox scar, M129-GP35-PtetCre Δ1kbmpn440::lox scar, M129-GP35-PtetCre Δ1kbmpn583::lox scar), a clone carrying a 30 kb deletion (M129-GP35-PtetCre Δ30kbNE region::pLoxPuro) and a clone carrying a 5.5 kb deletion that was complemented with the two essential genes found in this area (M129-GP35 Δ5.5kbmpn633-mpn638::mpn636-637lox scar)