Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Adaptation strategies of Pseudothermotoga species to hydrostatic pressure by transcriptional analyses

ABSTRACT: Adaptation strategies of Pseudothermotoga species to hydrostatic pressure by transcriptional analyses

PROVIDER: PRJNA938506 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Adaptation strategies of Pseudothermotoga species to hydrostatic pressure by transcriptional analyses

Project description:Pseudothermotoga elfii strain DSM9442 and P. elfii subsp. lettingae strain TMOT are hyperthermophilic bacteria. P. elfii is a moderate piezophile, isolated from an oil-producing well in Africa at a depth of more than 1600 m. P. lettingae is piezotolerant, isolated from a thermophilic bioreactor fed with methanol as the sole carbon and energy source. In this study, we analysed these bacteria at the genomic and transcriptomic levels. According to the hydrostatic pressure growth conditions the transcriptomic analyses revealed differentially expressed genes emphasizing amino acid and sugars metabolism and transport as the major hydrostatic pressure responding processes. Notably, this work highlights the central role of the amino acid aspartate as key intermediate of the pressure adaptation mechanisms of the deep strain P. elfii DSM 9442. In addition, several differentially expressed genes involved in the membrane and cell wall biosynthesis pathways may be linked to the chain formation morphotype previously described at high pressure for strain P. elfii DSM9442.

2023-03-30 | GSE226101 | GEO

High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii and piezosensible Pyrococcus furiosus

Project description:Pyrococcus yayanosii CH1 is the first and only obligate piezophilic hyperthermophilic microorganism discovered so far, that extends the physical and chemical limits of life on Earth and strengthens the idea of the existence of a hyperthermophilic biosphere in the depth of our planet. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses where performed in order to study the mechanisms implemented by the cell to face high hydrostatic pressure variations. In silico analyses showed that P. yayanosii genome is highly adapted to its harsh environment with precisely a loss of aromatic amino acid biosynthesis and the high constitutive expression of the energy metabolism compared to others non obligate piezophilic Pyrococus. Differential proteomics and transcriptomics analyses identified key hydrostatic pressure responsive genes involved in translation, chemotaxis, energy metabolism (hydrogenases and formate metabolism) and CRISPR-cas. Cells were grown at different hydrostatic pressures (20, 52 and 80 Mpa for P. yayanosii and 0.1 and 45 Mpa for P. furiosus) until they reached the middle of the exponential phase. Each culture was done 3 times independantly.

2015-09-09 | E-GEOD-72783 | biostudies-arrayexpress

High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii and piezosensible Pyrococcus furiosus

2015-09-09 | GSE72783 | GEO

Mouse oocytes: High hydrostatic pressure (HP) treated vs. Control

2012-01-06 | GSE28410 | GEO

Mouse oocytes: High hydrostatic pressure (HP) treated vs. Control

Project description:Transcription profiling of mouse oocytes treated with 20 MPa hydrostatic pressure for 60 minutes at 37 °C comparing control oocytes kept under identical conditions as pressure treated ones, except HHP treatment. One-condition experiment, HP treated oocytes vs. Control oocytes. Biological replicates: 4 HP treated replicates, 4 control replicates.

2012-01-06 | E-GEOD-28410 | biostudies-arrayexpress

Thermococcus eurythermalis A501, high hydrostatic pressure

Project description:In this study, we performed a global quantitative proteomic analysis under extreme temperatures, pH, hydrostatic pressure (HP) and salinity on an archaeal strain, Thermococcus eurythermalis A501. Here is the result of pressure adaptation: HP (40 MPa) tested under 85°C and 95°C, and the optimal culture condition (85°C, pH 7, 2.3% NaCl, 10 MPa) was used as the control.

2020-09-09 | PXD019007 | Pride

The effect of hydrostatic pressure in C. elegans

Project description:We examined whether hydrostatic-pressure induced nuclear DAF-16 functions as a transcription factor. The expression changes were monitored by using DNA microarray analyses, after the WT adult hermaphrodites were exposed to a pressure of ≥30 MPa for 5 minutes. The results showed significant and reproducible increase of 31 genes after 30 minutes

2020-07-31 | GSE133703 | GEO

Several genes expression patterns in Desulfovibrio hydrothermalis sp. nov. AM13T in response to hydrostatic pressure

Project description:We have identified differentially expressed genes according to hydrostatic pressure growth conditions in Desulfovibrio hydrothermalis. The transcriptomic datasets report the molecular mechanisms which could be involved in such adaptation and give information for the piezophile sulfate-reducing bacteria research communities. The data obtained pointed out a gradual response of D. hydrothermalis to an increase of hydrostatic pressure, with a threshold above 10 MPa and the involvement of a quite limited number of genes and/or pathways involved in the adaptation to hydrostatic pressure.

2015-10-13 | GSE55745 | GEO

Whole transcriptomic analysis of mouse ATDC5 chondrocyte precursor cells under 25 MPa hydrostatic pressure for 0, 1 , 4 or 24h

Project description:Hydrostatic pressure is one of the main mechanical stimuli cartilage cells are submitted to during joint loading. If moderate hydrostatic pressure is known to be beneficial to cartilage differentiation, excessive pressure, on the other hand, induces changes in cartilage similar to those observed in osteoarthritic cartilage. Therefore, the purpose of the experiment is to identify new target genes of high hydrostatic pressure in chondrocyte precursor cells.

2017-12-02 | E-MTAB-5326 | biostudies-arrayexpress

Mouse oocytes and in vitro fertilized four-cell stage embryos: High hydrostatic pressure (HP) treated vs. Control

Project description:This SuperSeries is composed of the following subset Series: GSE28410: Mouse oocytes: High hydrostatic pressure (HP) treated vs. Control GSE28411: Mouse in vitro fertilized four-cell stage embryos: High hydrostatic pressure (HP) treated vs. Control Refer to individual Series

2012-01-06 | E-GEOD-28443 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data