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Effect of depletion of MED23 on genome-wide occupancy of Sp1 and P300 during mouse OPC differentiation [cut&tag]

ABSTRACT: Effect of depletion of MED23 on genome-wide occupancy of Sp1 and P300 during mouse OPC differentiation [cut&tag]

PROVIDER: PRJNA938771 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23620728_1.fastq.gz	Fastqsanger.gz
	SRR23620728_2.fastq.gz	Fastqsanger.gz
	SRR23620729_1.fastq.gz	Fastqsanger.gz
	SRR23620729_2.fastq.gz	Fastqsanger.gz
	SRR23620730_1.fastq.gz	Fastqsanger.gz

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Effect of depletion of MED23 on genome-wide occupancy of Sp1 and P300 during mouse OPC differentiation [cut&tag]

Project description:To determine the role played by Med23 in the cooperation between Sp1 and P300.

2024-10-23 | GSE226123 | GEO

Effect of depletion of MED23 on gene expression during primary mouse OPC differentiation [RNA-seq]

Project description:To determine the role played by Med23 in OPC differentiation, OPCs were isolated from the Med23cKO (i.e., Med23-/-OPCs) or control mice (i.e., Med23+/+OPCs) and induced to differentiate in vitro. We then performed gene expression profiling analysis using data obtained from RNA-seq of Med23+/+iOLs and Med23-/-iOLs .

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Effect of depletion of MED23 on H3K27AC histone modifications during mouse OPC differentiation [ChIP-seq]

Project description:To determine the role played by Med23 in the genome wide H3K27AC histone modifications.

2024-10-23 | GSE226121 | GEO

Effect of depletion of MED23 on gene expression during primary mouse OPC differentiation [RNA-seq]

Project description:Effect of depletion of MED23 on gene expression during primary mouse OPC differentiation [RNA-seq]

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Effect of depletion of MED23 on H3K27AC histone modifications during mouse OPC differentiation [ChIP-seq]

Project description:Effect of depletion of MED23 on H3K27AC histone modifications during mouse OPC differentiation [ChIP-seq]

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Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

Project description:Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recombinant Mediator subunit MED23 but not to several other Mediator components. One of these factors, hnRNP L, specifically interacts with MED23 in vitro and in vivo. Consistently, Mediator partially colocalizes with hnRNP L and the splicing machinery in the cell. Functionally Med23 regulates a subset of hnRNP L-targeted alternative splicing (AS) and alternative cleavage and polyadenylation (APA) events as shown by minigene reporters and exon array analysis. ChIP-seq analysis revealed that Med23 can regulate hnRNP L occupancy at their co-regulated genes. Taken together, these results demonstrate a crosstalk between Mediator and the splicing machinery, suggesting a novel mechanism for coupling mRNA processing to transcription. Examination of hnRNP L and H3K36me3 enrichment in sictrl and si23 Hela cells

2012-02-24 | E-GEOD-33887 | biostudies-arrayexpress

Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

Project description:Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recombinant Mediator subunit MED23 but not to several other Mediator components. One of these factors, hnRNP L, specifically interacts with MED23 in vitro and in vivo. Consistently, Mediator partially colocalizes with hnRNP L and the splicing machinery in the cell. Functionally Med23 regulates a subset of hnRNP L-targeted alternative splicing (AS) and alternative cleavage and polyadenylation (APA) events as shown by minigene reporters and exon array analysis. ChIP-seq analysis revealed that Med23 can regulate hnRNP L occupancy at their co-regulated genes. Taken together, these results demonstrate a crosstalk between Mediator and the splicing machinery, suggesting a novel mechanism for coupling mRNA processing to transcription. We performed an exon array experiment using HeLa cells expressing Med23, hnRNP L or control siRNAs which were established by a virus-mediated siRNA technology. Each sample was done in three biological replicates. Total RNA of these cell lines was processed and hybridized to the Affymetrix human exon array.

2012-02-24 | E-GEOD-33771 | biostudies-arrayexpress

Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

2012-02-24 | GSE33887 | GEO

Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

2012-02-24 | GSE33771 | GEO

Med12 and p300 chips in HSPCs

Project description:We characterized the genome wide occupancy of Med12 and p300 in mouse HSPCs. We also characterize p300 occupancy upon shRNA against control or Med12. ChIP-seq analysis of Med12 and/or p300 in untreated HSPCs

2016-08-25 | E-GEOD-76053 | biostudies-arrayexpress

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