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Effect of depletion of MED23 on genome-wide occupancy of Sp1 and P300 during mouse OPC differentiation [cut&tag]

ABSTRACT: Effect of depletion of MED23 on genome-wide occupancy of Sp1 and P300 during mouse OPC differentiation [cut&tag]

PROVIDER: PRJNA938771 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23620728_1.fastq.gz	Fastqsanger.gz
	SRR23620728_2.fastq.gz	Fastqsanger.gz
	SRR23620729_1.fastq.gz	Fastqsanger.gz
	SRR23620729_2.fastq.gz	Fastqsanger.gz
	SRR23620730_1.fastq.gz	Fastqsanger.gz

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Effect of depletion of MED23 on genome-wide occupancy of Sp1 and P300 during mouse OPC differentiation [cut&tag]

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Effect of depletion of MED23 on gene expression during primary mouse OPC differentiation [RNA-seq]

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Effect of depletion of MED23 on H3K27AC histone modifications during mouse OPC differentiation [ChIP-seq]

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Effect of depletion of MED23 on gene expression during primary mouse OPC differentiation [RNA-seq]

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Project description:To analysis TCR induced Med23-dependent gene expression programs in T cells, we have employed microarray expression profiling as a discovery platform to identify genes with the potential to be regulated by Med23 in unstimulated or stimulated T cells. Mice CD4 T cells from wildtype and Med23 knockout mice were left unstimulated or stimulated for 6 hr, and gene expression difference was identified among four samples. Expression of seven genes (Med23, IFNg, IL2, Egr2, c-Fos, c-Jun and Foxp1) was validated in the same RNA samples by real-time PCR. TCR induced Med23-dependent gene expression was measured after unstimulation or stimulation for 6 hr in wildtype and Med23 knockout CD4 T cells.

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LPC 18:1 protein interaction (OPC)

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