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Single-cell RNA sequencing reveals the local cell landscape in mouse epididymal initial segment during aging

ABSTRACT: Single-cell RNA sequencing reveals the local cell landscape in mouse epididymal initial segment during aging

PROVIDER: PRJNA939630 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23641933_1.fastq.gz	Fastqsanger.gz
	SRR23641933_2.fastq.gz	Fastqsanger.gz
	SRR23641934_1.fastq.gz	Fastqsanger.gz
	SRR23641934_2.fastq.gz	Fastqsanger.gz

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Similar Datasets

Single-cell RNA sequencing reveals the local cell landscape in mouse epididymal initial segment during aging

Project description:Introduction: Morphological and functional alternations of the aging reproductive organs result in decreased male fertility. We previously reported that the epididymal initial segment underwent remarkable histological changes and chronic immune-activation in mouse during aging. However, the local cell landscape in the aged epididymal initial segment are poorly understood. Objectives: We aimed to characterize the aging-associated cellular and molecular features of mouse epididymal initial segment.

2023-04-23 | GSE226310 | GEO

RNA sequencing analysis of Ovch2 knockout initial segment-caput epididymis.

Project description:Purpose: The purpose of this study is to characterize gene expression in the Ovch2-ablated mouse initial segment (IS)-caput epididymis. Methods: Ovch2-/- mice were used when they were 14-week-old. IS-caput epididymal mRNA profiles were generated by deep sequencing, in triplicate, using Illumina NovaSeq6000. Results: RNA-seq data identified differentially expressed transcripts. Conclusions: Our results show that the expression of many genes was not critically affected by Ovch2 ablation.

2024-05-01 | GSE265964 | GEO

RNA sequencing analysis of busulfan-treated initial segment-caput epididymis.

Project description:Purpose: The purpose of this study is to characterize genes whose expressions in the initial segment (IS)-caput epididymis were affected by busulfan intraperitoneal injection. Methods: 8-week-old wild-type mice were treated with single intraperitoneal injection of dimethyl sulfoxide (DMSO) or busulfan (40mg/kg body weight) disolved in DMSO and mice were left for 4 weeks until tissue sampling at 12 weeks old. IS-caput epididymal mRNA profiles were generated by deep sequencing, in triplicate, using Illumina NovaSeq6000. Results: RNA-seq data identified differentially expressed transcripts. Conclusions: Our results show that the expression of many genes was affected by busulfan injection.

2024-02-18 | GSE247820 | GEO

RNA sequencing analysis of unilateral orchidectomy, bilateral orchidectomy, and Nell2 knockout initial segment-caput epididymis.

Project description:Purpose: The purpose of this study is to characterize genes whose expressions in the initial segment (IS)-caput epididymis were affected by unilateral orchidectomy (UOD), bilateral orchidectomy (BOD), and Nell2 gene ablation. Methods: The unilateral or bilateral orchidectomy of WT mice was performed from 8 weeks old and continued for 4 weeks until tissue sampling at 12 weeks old. Contralateral epididymis was used as a control for UOD. Sham operation was also done as a control for BOD. Nell2-/- mice and their control WT mice were used wthen they were 14-week-old. IS-caput epididymal mRNA profiles were generated by deep sequencing, in triplicate, using Illumina NovaSeq6000. Results: RNA-seq data identified differentially expressed transcripts. Conclusions: Our results show that the expression of many genes was affected by UOD, BOD, and Nell2 ablation.

2023-11-19 | GSE247764 | GEO

Testicular lumicrine factors regulate ERK, STAT and NFKB pathways in the initial segment of the rat epididymis to prevent apoptosis

Project description:The initial segment of the epididymis is vital for male fertility, therefore, it is important to understand the mechanisms that regulate this important region. Deprival of testicular luminal fluid factors/lumicrine factors from epididymis, a subset of cells within the initial segment undergo apoptosis. In this study, microarray analyses was used to examine early changes in the downstream signal transduction pathways following the loss of lumicrine factors, and we discovered the following cascade of events leading to loss of protection and eventual apoptosis. First, mRNA expression of several key components of ERK pathway decreased sharply after 6 hours of loss protection from testicular lumicrine factors. After 12 hours, the levels of mRNA expression of STAT and NF-кB pathways components increased, mRNA expression of genes encoding cell cycle inhibitors increased. After 18 hours of loss protection from testicular lumicrine factors, apoptosis was observed in the initial segment. In conclusion, testicular lumicrine factors protect the cells of the initial segment by activating ERK pathway, repressing STAT and NF-кB pathways, and preventing a cascade of reactions leading to apoptosis. Unilateral efferent duct ligation (EDL) surgeries were performed to block lumicrine testicular fluid factors from reaching one side of epididymis. For the control, a sham operation was performed on the contra-lateral side within the same animal. After 6, 12 and 18 hours of EDL, the most proximal region of the initial segment containing zone 1a and 1b of initial segment was dissected out. In this microarray study, sham control and each time point of EDL (sham, 6h EDL, 12h EDL, 18h EDL) all have 4 replicates, a total of 16 arrays were hybridized.

2011-03-11 | E-GEOD-21806 | biostudies-arrayexpress

Testicular lumicrine factors regulate ERK, STAT and NFKB pathways in the initial segment of the rat epididymis to prevent apoptosis

2011-03-11 | GSE21806 | GEO

PTEN is essential in the maintenance of normal transporter activity in the epididymal epithelium

Project description:A fully developed initial segment, the most proximal region of the epididymis, is important for male fertility. Conditional deletion of Pten from the initial segment from postnatal day 17 onwards resulted in de-differentiation of the initial segment. When spermatozoa progressed through the de-differentiated epididymal duct, they developed angled flagella, suggesting compromised sperm maturation, which eventually resulted in male infertility. To understand mechanisms that underlie this sperm maturation defect following loss of Pten, we compared transcriptome profile of the initial segment between controls and knockouts and revealed that the transporter activity was one of the top molecular and cellular functions altered following loss of Pten. Alteration in the protein levels and localization of several transporters following loss of Pten were also observed by immunofluorescence analysis. Epithelial cells of the initial segment from knockouts are more permeable to Fluorescein isothiocyanate-dextran (4000da) compared to controls. When analyzing GEO datasets with Pten deletion in the prostate and inner ear of mice, alteration in transporter activity was also found in these Pten knockout mouse models, suggesting it is a common phenomenon. PTEN regulates transporter activities probably through regulation of cell shape, size, and membrane property, which in turn regulates the intracellular and intercellular micro-environment.

2019-12-19 | GSE92614 | GEO

RNA sequencing analysis of Ovch2 knockout initial segment-caput epididymis.

Project description:RNA sequencing analysis of Ovch2 knockout initial segment-caput epididymis.

| PRJNA1105058 | ENA

RNA sequencing analysis of busulfan-treated initial segment-caput epididymis.

Project description:RNA sequencing analysis of busulfan-treated initial segment-caput epididymis.

| PRJNA1040783 | ENA

Immunoproximity biotinylation reveals the axon initial segment proteome

Project description:The axon initial segment (AIS) is a specialized neuronal compartment required for action potential generation and neuronal polarity. However, understanding the mechanisms regulating AIS structure and function has been hindered by an incomplete knowledge of its molecular composition. Here, using immuno-proximity biotinylation we further define the AIS proteome and its dynamic changes during neuronal maturation. Among the many AIS proteins identified, we show that SCRIB is highly enriched in the AIS both in vitro and in vivo, and exhibits a periodic architecture like the axonal spectrin-based cytoskeleton. We found that ankyrinG interacts with and recruits SCRIB to the AIS. However, loss of SCRIB has no effect on ankyrinG. This powerful and flexible approach further defines the AIS proteome and provides a rich resource to elucidate the mechanisms regulating AIS structure and function.

2024-01-02 | PXD045921 | Pride

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