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Transcriptomic analysis the mechanism of detoxification metabolism induced by different insecticide in Spodoptera frugiperda

ABSTRACT: Transcriptomic analysis the mechanism of detoxification metabolism induced by different insecticide in Spodoptera frugiperda

PROVIDER: PRJNA940659 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23693754_1.fastq.gz	Fastqsanger.gz
	SRR23693754_2.fastq.gz	Fastqsanger.gz
	SRR23693755_1.fastq.gz	Fastqsanger.gz
	SRR23693755_2.fastq.gz	Fastqsanger.gz
	SRR23693756_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis the mechanism of detoxification metabolism induced by different insecticide in Spodoptera frugiperda

Project description: In order to clarify the molecular mechanism of metabolic detoxification in Spodoptera frugiperda, the enzyme activity assay and transcriptome sequencing analysis were used to screen and identify the relevant genes related to metabolic detoxification after induced by insecticides at LC50 dose.

2023-03-08 | GSE226546 | GEO

MicroRNAs Mediate Insecticide Tolerance in Spodoptera frugiperda

Project description:Spodoptera frugiperda is the world’s major agricultural pests, and has the distinctive features of high fecundity, strong migratory capacity and high resistance to most insecticides. At present, the control of S. frugiperda in China relies mainly on the spraying of chemical insecticides. MicroRNAs (miRNAs) are a class of small, single-stranded, non-coding RNAs, and play crucial regulatory roles in various physiological processes, including the insecticide resistance in insects. However, little is known about the regulatory roles of miRNAs on the resistance of S. frugiperda to insecticides. In the present research, the miRNAs that were differentially expressed after cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole treatment were analyzed by RNA-Seq. A total of 504 miRNAs were systematically identified from S. frugiperda, and 24, 22, 31 and 30 miRNAs were differentially expressed after treatments of cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole. GO and KEGG enrichment analyses were used to predict the function of differentially expressed miRNAs’ target genes. Importantly, ten miRNAs were significantly differentially expressed among the treatments of three insecticides. MiR-278-5p, miR-13b-3p, miR-10485-5p and miR-10483-5p were significantly down-regulated among the treatments of three insecticides by RT-qPCR. Furthermore, overexpression of miR-278-5p, miR-13b-3p, miR-10485-5p and miR-10483-5p significantly increased the mortality of S. frugiperda to cyantraniliprole and emamectin benzoate. The mortality was significantly increased with spinetoram treatment after overexpression of miR-13b-3p, miR-10485-5p and miR-10483-5p. These results suggest that miRNAs, which are differentially expressed in response to insecticides, may play a key regulatory role in the insecticide resistance in S. frugiperda.

2021-12-04 | GSE189968 | GEO

Identification of differentially expressed miRNAs associated with diamide detoxification pathways in Spodoptera frugiperda

Project description:The fall armyworm (FAW) Spodoptera frugiperda is one of the most severe economic pests of multiple crops globally. Control of this pest is often achieved using insecticides; however, over time, S. frugiperda has developed resistance to new mode of action compounds, including diamides. Previous studies have indicated diamide resistance is a complex developmental process involving multiple detoxification genes. Still, the mechanism underlying the possible involvement of microRNAs in post-transcriptional regulation of resistance has not yet been elucidated. In this study, a global screen of microRNAs (miRNAs) revealed 109 known and 63 novel miRNAs. Nine miRNAs (four known and five novel) were differentially expressed between insecticide-resistant and -susceptible strains. Gene Ontology analysis predicted putative target transcripts of the differentially expressed miRNAs encoding significant genes belonging to detoxification pathways. Additionally, miRNAs are involved in response to diamide exposure, indicating they are probably associated with the detoxification pathway. Thus, this study provides comprehensive evidence for the link between repressed miRNA expression and induced target transcripts that possibly mediate diamide resistance through post-transcriptional regulation. These findings highlight important clues for further research to unravel the roles and mechanisms of miRNAs in conferring diamide resistance.

2023-09-01 | GSE233976 | GEO

MicroRNAs Mediate Insecticide Tolerance in Spodoptera frugiperda

Project description:MicroRNAs Mediate Insecticide Tolerance in Spodoptera frugiperda

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RNA-Sequencing of 3rd-instar Spodoptera frugiperda post-infection with Spodoptera frugiperda ascovirus-1a (SfAV-1a), the ascovirus type species.

Project description:Purpose: We analyzed the 3rd-instar Spodoptera frugiperda response after SfAV-1a infection. Specifically, we targeted three gene types in the infected host namely, mitochondrial, cytoskeleton and innate immunity genes.

2020-02-09 | GSE114901 | GEO

Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome

Project description:Comparison of the effects of 2 polydnaviruses (HdIV vs MdBV) injection on L5 Spodoptera frugiperda larvae hemocytes transcriptome.

2011-04-05 | GSE16775 | GEO

Comparison of gene expression in three strains of Spodoptera Frugiperda (Insecta: Lepidoptera: Noctuidae)

Project description:The aim of this experiment was to compare the transciptome of the fall armyworm (Spodoptera frugiperda) strain SUS (a laboratory insecticide-susceptible standard) with organophosphate (OP) and pyrethroid (PYR) resistant strains were collected in cornfields located in Minas Gerais and Mato Grosso States, Brazil, in 2008 and maintained in the laboratory under selection with chlorpyrifos (at increasing discriminating doses from 100 up to 400 µg of insecticide/g of insect in a topical bioassay) or lambda-cyhalothrin (from 8.4 up to 27 µg of insecticide/g of insect) respectively The custom microarray used in this study was designed using the Agilent eArray platform (Agilent Technologies). A SurePrint HD (8×15k) expression array was designed using the base composition and the best probe methodologies to design sense orientation 60-mer probes with a 3′ bias. The FAW EST database (SPODOBASE) was used as the reference transcriptome (Negre, Hotelier et al. 2006). These sequences are derived from 8 cDNA libraries as follows: Sf1F: Fat body, Sf1H: Hemocyte, Sf1M: Midgut, Sf1P: Pool of various tissues, Sf2H: Immune Challenged hemocytes, Sf2L: Sf21 Cell lines sequences, Sf2M: Xenobiotic Induced Midgut and Sf9L: Sf9 cell lines sequences. All assembled contigs and singlets were kindly sent by the website maintainers. The BLAST2GO software v.2.3.1 (http://www.blast2go.org) was used to annotate the EST database. 60-mer probes were designed for all 7,552 assembled contigs and 5,519 annotated singlets (BlastX), totaling 13,071 sequences. For contigs encoding detoxification enzymes (P450s, GSTs and CEs) three probes were designed. Additional probe groups for 15 control genes were also included. For reference all sequences are included in the zip file with array data. References: Negre, V., T. Hotelier, et al. (2006). "SPODOBASE : an EST database for the lepidopteran crop pest Spodoptera." BMC Bioinformatics 7: 322.

2013-08-01 | GSE43295 | GEO

Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte and fat body transcriptomes

Project description:This SuperSeries is composed of the following subset Series: GSE16775: Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome GSE16776: Effect of HdIV or MdBV injection on the Spodoptera frugiperda fat body transcriptome Refer to individual Series

2011-04-05 | E-GEOD-17293 | biostudies-arrayexpress

Effect of HdIV or MdBV injection on the Spodoptera frugiperda fat body transcriptome

Project description:Comparison of the effects of 2 polydnaviruses (HdIV vs MdBV) injection on L5 Spodoptera frugiperda larvae fat body transcriptome.

2011-04-05 | GSE16776 | GEO

Spodoptera frugiperda

Project description:Spodoptera frugiperda Metagenome

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