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Exonuclease Xrn1 regulates TORC1 signaling in response to SAM availability

ABSTRACT: Exonuclease Xrn1 regulates TORC1 signaling in response to SAM availability

PROVIDER: PRJNA941789 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23724456_1.fastq.gz	Fastqsanger.gz
	SRR23724456_2.fastq.gz	Fastqsanger.gz
	SRR23724457_1.fastq.gz	Fastqsanger.gz
	SRR23724457_2.fastq.gz	Fastqsanger.gz
	SRR23724458_1.fastq.gz	Fastqsanger.gz

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Exonuclease Xrn1 regulates TORC1 signaling in response to SAM availability

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Project description:The main cytoplasmic mRNA decay pathway in yeast uses the 5’-3’ exonuclease Xrn1 (Parker and Song, Nat Struct Mol Biol. 2004 ). This protein shuttles from the cytoplasm to the nucleus, where it has a role as transcription factor (Haimovich et al. Cell 2013). In this work, we find that most of the global phenotypes of an xrn1 mutant are partially complemented by a cytoplasmic version of the paralogous 5’-3’exonuclease Rat1 (cRat1) indicating that this 5’-3’-exonuclease has a similar enzymatic capacity as Xrn1. The lack of a cytoplasmatic 5’-3’-exoribonuclease is the cause of the physiological defects of an xrn1 mutant. The capacity of cRat1 to perform co-translational decay is, however, very limited. The comparison with the strain having a NLS1∆-NLS2∆-Xrn1 version shows that it is slightly deficient in 5’→3’-co-translational decay but much more efficient than cRat1. In both strains, cRat1 and -Xrn1-NLS1&2, the lack of nuclear Xrn1 has a very minor influence on cell growth.

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Xrn1 activates transcription, translation and decay of mRNAs encoding membrane proteins

Project description:To study the role of the exonuclease Xrn1 in translational control, we performed ribosome profiling and RNA-seq in Xrn1-depleted cells. By using an auxin-inducible degron, we were able to study immediate effects of Xrn1 depletion in translational control. Therefore, we could overcome experimental limitations associated to stable deletion mutants.

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Xrn1 modulates the dynamic osmostress response at a transcriptional and translational level

Project description:To study the role of the exonuclease Xrn1 in gene expression dynamics under osmotic stress conditions, we performed RNA-seq in Xrn1-depleted cells. By using an auxin-inducible degron, we were able to study immediate effects of Xrn1 depletion in gene expression dynamics. Therefore, we could overcome experimental limitations associated to stable deletion mutants.

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Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast

Project description:mRNA degradation is one of the main steps of gene expression, and a key player is the 5’-3’ exonuclease Xrn1. In Saccharomyces cerevisiae, it was previously shown, by a microscopy approach, that Xrn1 is located to different cellular compartments, depending on physiological state. During exponential growth, Xrn1 is distributed in the cytoplasm, while it is present in the eisosomes after the post-diauxic shift (PDS). Here, we biochemically characterized the Xrn1-associated complexes in different cellular states. We demonstrate that, after PDS, Xrn1 but not the decapping (DCP), nor Lsm1-7/Pat1 complexes, was sequestered in the eisosomes, thus preserving mRNAs from degradation.

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Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast (2)

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