Single-cell transcriptomic analyses of pancreatic islets reveals islet cell-type-specific adaptations to pregnancy and the postpartum
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ABSTRACT:
PROVIDER: PRJNA941793 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR23724251_1.fastq.gz | Fastqsanger.gz | |||
| SRR23724251_2.fastq.gz | Fastqsanger.gz | |||
| SRR23724252_1.fastq.gz | Fastqsanger.gz | |||
| SRR23724252_2.fastq.gz | Fastqsanger.gz | |||
| SRR23724253_1.fastq.gz | Fastqsanger.gz |
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Project description:Pancreatic β-cell mass expands during pregnancy and regresses in the postpartum period in conjunction with dynamic metabolic demands on maternal glucose homeostasis. To understand transcriptional changes driving these adaptations in β-cells and other islet cell types, we performed single-cell RNA sequencing on islets from virgin, late gestation, and early postpartum mice. We identified transcriptional signatures unique to gestation and the postpartum in β-cells, including induction of the AP-1 transcription factor subunits and other genes involved in the immediate-early response (IEGs). Additionally, we found pregnancy and postpartum-induced changes differed within each endocrine cell type, and in endothelial cells and macrophages within islets. Together, our data reveal novel insights into cell-type specific transcriptional changes responsible for adaptations by islet cells to pregnancy and their resolution postpartum.
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