Project description:Purpose: The purpose of this study was to investigate the effect of quorum sensing-related luxS gene on the transcription level of Klebsiella pneumoniae FK6768. Methods: We constructed the luxS gene knockout strain of FK6768 and its complement strain, and performed transcriptome sequencing.
Project description:The increasing antibiotic resistance of Klebsiella pneumoniae poses a serious threat to global public health. To investigate the antibiotic resistance mechanism of Klebsiella pneumonia, we performed gene expression profiling analysis using RNA-seq data for clinical isolates of Klebsiella pneumonia, KPN16 and ATCC13883. Our results showed that mutant strain KPN16 is likely to act against the antibiotics through increased increased butanoate metabolism and lipopolysaccharide biosynthesis, and decreased transmembrane transport activity.
