Project description:Gene expression in the obligatory aerobic acetic acid bacterium Gluconobacter oxydans was shown to respond to oxygen limitation, but the regulators involved are unknown. In this study, we analyzed the function of a transcriptional regulator named GoxR, which belongs to the FNR family. Here, we aplied RNAseq to identify target genes of GoxR by comparing genes expression of a delta-goxR and its parental strain after 20 min of oxygen limitation.
Project description:We reported that analysis of PQQ metabolism in Gluconobacter oxydasns strain at gene expression level. Acoording to present study that pqqB gene can strengthen the transport of PQQ. Wild type strain and pqqB-overexpression strain were compared to find other candidate key genes which can encoded proteins are involved in PQQ transport. Results provide important information of the response of pqqB-overexpression, such as genes encoded membrane proteins and other indirectly functional genes.
