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MTOR pathway inhibition attenuates hypercapnia-induced autophagy arrest and dysfunctional myogenesis

ABSTRACT: mTOR pathway inhibition attenuates hypercapnia-induced autophagy arrest and dysfunctional myogenesis

PROVIDER: PRJNA942276 | ENA |

REPOSITORIES: ENA

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mTOR pathway modulation improves hypercapnia-induced satellite cell autophagy arrest and dysfunctional myogenesis.

Project description:Chronic CO2 retention, or hypercapnia, occurs in pulmonary diseases and leads to skeletal muscle wasting. Muscle wasting and hypercapnia are independently associated with substantial mortality. While abnormal skeletal muscle stem -satellite- cells, contribute to impaired myogenesis in pulmonary diseases, no mechanistic data exists on hypercapnia-driven dysfunctional satellite cells and myogenesis in-vivo. Autophagy regulates satellite cell activation and myogenesis, and while elevated CO2 inhibits autophagy in inflammatory cells, it has never been evaluated in the context of hypercapnia-driven abnormal myogenesis. Using lineage tracing in pre- and post-stem cell transplantation experiments, we show that hypercapnia undermines both Pax7- and α-7 integrin-expressing satellite cells activation, replication and myogenesis. Satellite cells’ multi-omic analyses indicate that hypercapnia disrupts multiple pathways regulated by autophagy. Moreover, autophagy-specific LC3 fluorescence-reporting mouse and cellular data demonstrate that hypercapnia reduces expressions of AMPK and Ulk1, which critically participate in autophagosome formation, and has no effect on the antagonistic mTOR pathway including Ulk1 serine-757 phosphorylation. Moreover, Atg7 knockout-driven loss of autophagy function phenocopies the effects on hypercapnia. After rapamycin administration, the hypercapnia-induced myogenic deficit is corrected by a sharp AMPK activation leading to the autophagy-stimulatory Ulk1 phosphorylation at serine-555, autophagy acceleration, increased satellite cells replication and improved myogenesis post-satellite cells transplantation.

2025-01-15 | GSE266996 | GEO

mTOR pathway modulation improves hypercapnia-induced satellite cell autophagy arrest and dysfunctional myogenesis.

Project description:mTOR pathway modulation improves hypercapnia-induced satellite cell autophagy arrest and dysfunctional myogenesis.

| PRJNA1109313 | ENA

mTOR pathway inhibition attenuates hypercapnia-induced autophagy arrest and dysfunctional myogenesis

Project description:Hypercapnia exposure in mice leads to reduced skeletal muscle repair capacity and myogenic potential. Autophagy is also reduced in this context. Satellite cells from mice treated with hypercapnia were isolated and compared to satellite cells from normocapnia control mice. Cells were isolated by FACS using Pax7-GFP tamoxifen inducible reporter to ensure enrichment of Pax7 cells.

2025-01-15 | GSE226928 | GEO

Buffered hypercapnia alters the transcriptional profile in monocytes.

Project description:In monocytes we used RNA-sequencing to investigate the effect of 4hrs of exposure to buffered hypercapnia (10% CO2) compared to normocapnia (5% CO2) with and without the pro-inflammatory stimulus LPS (2.5ug/ml) for the final 2hrs. Buffered hypercapnia causes transcriptional changes associated with altered metabolic function in both the basal and stimulated states.

2023-04-07 | GSE206333 | GEO

Drosophila elevated CO2 (hypercapnia) microarray

Project description:The effects of elevated CO2 (hypercapnia) on organisms are not well known, nor are the molecular pathways by which organisms sense CO2. We have performed microarray analysis on Drosophila in order to develop a genetic model for better understanding the effects of CO2. Equal numbers of 5-day old male and female flies (at least 40 total) were exposed to 13% CO2 or air for 24h and RNA extracted. Gene expression changes in CO2 compared with air were analyzed. Experiments were performed in triplicate and the 3 separate microarray experiments are included.

2010-06-25 | E-GEOD-17444 | biostudies-arrayexpress

Drosophila elevated CO2 (hypercapnia) microarray

2009-09-30 | GSE17444 | GEO

NR4A2 and NR4A3 selectively modulate elements of the monocyte response to buffered hypercapnia

Project description:In wild type, NR4A2-deficient, and NR4A3-deficient monocytes we used RNA-sequencing to investigate the effect of 4hrs of exposure to buffered hypercapnia (10% CO2) compared to normocapnia (5% CO2) with and without the pro-inflammatory stimulus LPS (2.5ug/ml) for the final 2hrs. Buffered hypercapnia causes transcriptional changes associated with altered metabolic function in both the basal and stimulated states.

2024-03-20 | GSE251925 | GEO

Effect of Hypercapnia on Murine Ventilator-Induced Lung Injury

Project description:Addition of CO2 to the inspired gas can ameliorate lung injury during high tidal volume mechanical ventilation in animal models. Although some effects of hypercapnia on physiology and cell signaling have been characterized, we hypothesized that assessment of genome-wide gene expression patterns would reveal novel pathways of protection. We subjected male C57BL/6J mice to non-injurious low stretch (tidal vol = 10 mL/kg, PEEP = 2 cm H2O) or injurious high stretch (tidal volume approx 35 mL/kg, PEEP = 0 cm H2O) mechanical ventilation for 3 hours under normocapnia (FiCO2 = 0) or hypercapnia (FiCO2 = 0.12).

2017-02-22 | GSE86229 | GEO

mTOR

Project description:mTOR

| PRJEB9498 | ENA

Myeloid Zfhx3 Deficiency Protects Against Hypercapnia-induced Suppression of Host Defense Against Influenza A Virus

Project description:Zfhx3, human ortholog of the Drosophila Zfh2 and first known mediator of hypercapnic immune suppression in vivo, is expressed in human and mouse MØs and its myeloid deletion reversed the effects of hypercapnia in gene expression in murine alveolar macrophages and protects against influenza A virus proliferation, lung injury and mortality increased by hypercapnia in infected mice.

2024-01-21 | GSE183922 | GEO

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