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Transcriptome data from U2OS cells and hTERT CRISPR clones

ABSTRACT: Transcriptome data from U2OS cells and hTERT CRISPR clones

PROVIDER: PRJNA942325 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23753525_1.fastq.gz	Fastqsanger.gz
	SRR23753525_2.fastq.gz	Fastqsanger.gz
	SRR23753526_1.fastq.gz	Fastqsanger.gz
	SRR23753526_2.fastq.gz	Fastqsanger.gz
	SRR23753527_1.fastq.gz	Fastqsanger.gz

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U2OS cells and hTERT CRISPR clones

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Project description:CRISPR screen: U2OS or U2OS p53KO cells expressing Cas9 were transduced with a whole-genome library of CRISPR sgRNAs, then treated with either DMSO or etoposide. Differential sgRNA abundances were calculated for each condition and used to determine the effect of each single gene knockout on fitness and the drug-induced death rate. RNA-Seq: U2OS or U2OS p53KO cells were cultured with either DMSO or etoposide for 48 hours, and then U2OS cells were incubated in this conditioned media for 8 hours. RNA was collected and use to observe differential expression changes between conditions.

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RNAseq analysis of U2OS cell clones made to stably expressing exogenous CIITA

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hTERT promotes cell adhesion and migration independent of telomerase activity

Project description:We sequenced mRNA from 6 human cell lines stably over-expressed specific gene or empty vector, and searched for differently expressed genes after gene over-expression as compared to empty vector. mRNA levels were compared as following pairs: U2OS+hTERT vs U2OS+vector; U2OS+hTERTmut vs U2OS+vector; VA-13+hTERT vs VA-13+vector; VA-13+hTERTmut vs VA-13+vector.

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U2OS shE4F1 transcriptome

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