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Effect of depletion of FOXL2 on gene expression in KGN cells

ABSTRACT: Effect of depletion of FOXL2 on gene expression in KGN cells

PROVIDER: PRJNA942945 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23773321_1.fastq.gz	Fastqsanger.gz
	SRR23773321_2.fastq.gz	Fastqsanger.gz
	SRR23773323_1.fastq.gz	Fastqsanger.gz
	SRR23773323_2.fastq.gz	Fastqsanger.gz
	SRR23773325_1.fastq.gz	Fastqsanger.gz

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Effect of depletion of FOXL2 on gene expression in KGN cells

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Project description:In order to identify novel FOXL2 targets, we have transfected KGN cells using an expression vector containing the coding sequence of FOXL2 or, as a reference, the empty vector (mock transfection). The transcriptome perturbation induced by FOXL2 overexpression was then followed by DNA chips (i.e. FOXL2- vs. mock-transfected cells) using the platform developed by NimbleGen Systems, Inc.

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Gene knockdown and overexpression of 402C>G FOXL2 in COV434 and KGN cells

Project description:Despite their distinct biology, granulosa cell tumours (GCTs) are treated the same as other ovarian tumours. Intriguingly, a recurring somatic mutation in the transcription factor Forkhead Box L2 (FOXL2) 402C>G has been found in nearly all GCTs examined. This investigation aims to identify the pathogenicity of mutant FOXL2 by studying its altered transcriptional targets. The expression of mutant FOXL2 was reduced in the GCT cell line KGN, and wildtype and mutant FOXL2 were overexpressed in the GCT cell line COV434. Comparisons were made between the transcriptomes of control cells and cells altered by FOXL2 knockdown and overexpression, to detect potential transcriptional targets of mutant FOXL2. Comparisons were made between the transcriptomes of control cells and cells altered by FOXL2 knockdown and overexpression, to detect potential transcriptional targets of mutant FOXL2. Mutant FOXL2 was silenced in KGN cells with the use of siRNA and a non-targetting siRNA control. Wildtype and mutant FOXL2 were overexpressed in COV434 cells with the use of overexpression plasmids and an empty plasmid control. Each experiment was performed in triplicate. Total RNA was harvested at 24h post transfection and hybridised to Affymetrix U133 plus 2.0 arrays.

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Gene knockdown and overexpression of 402C>G FOXL2 in COV434 and KGN cells

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ChIP-chip by array of human adult ovarian granulosa cell tumor derived cell line KGN using anti-FOXL2 antibodies

Project description:ChIP-on-Chip experiment using chromatin from the human adult ovarian granulosa cell tumor derived cell line KGN (Nishi et al, 2001), or from a KGN subclone overexpressing WT FOXL2 (KF3 subclone), and an isovolumic blend of our custom anti-FOXL2 polyclonal antibodies (Cocquet J et al, 2002). Non precipitated sheared matched deproteinized chromatin (Input) was used a control to estimate enrichment peaks (and thus FOXL2 binding sites) from IPed DNA. DNA from three independent ChIP assays (Input extractions) was pooled, and 100ng of DNA was linearly amplified using the Whole Genome Amplification kit (Sigma). The two ChIP-chip experiments serve as biological replicates. Moreover, 42 out of 48 targets promoters chosen from the âenrichment peakâ list were subsequently confirmed as enrichment sites in anti-FOXL2 ChIPed DNA for subsequent experiments.

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Effect of p.Cys134Trp FOXL2 variant on gene expression in COV434 granulosa cell tumor derived cells

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Role of YAP/TAZ in KGN human granulosa cell line [Clariom_S_Human]

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Effect of depletion of Elovl6 on gene expression

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