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Assay for transposase-accessible chromatin-sequencing (ATAC-seq) of heart samples from Wtapflox/flox and Wtap-CKO mice, respectively.

ABSTRACT: Assay for transposase-accessible chromatin-sequencing (ATAC-seq) of heart samples from Wtapflox/flox and Wtap-CKO mice, respectively.

PROVIDER: PRJNA943503 | ENA |

REPOSITORIES: ENA

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Assay for transposase-accessible chromatin-sequencing (ATAC-seq) of liver samples in Wtapflox/flox and hepatocyte-specific Wtap knockout (Wtap-HKO) mice

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Single-nucleus transcriptome analysis of interscapular brown adipose tissue (iBAT) in Wtapflox/flox and BAT-specific Wtap knockout (BKO) mice

Project description:Purpose: Through Single-nucleus transcriptome analysis, we aim to characterize the changes of cell heterogeneity in iBAT obtained from Wtap-BKO mice compared with that in Wtapflox/flox mice. Methods: Nuclei were isolated from iBAT of Wtap flox/flox and Wtap-BKO mice at 8 weeks old. Each sample was pooled from 4 mice for each group. Conclusion: The Single-nucleus transcriptomes of iBAT in Wtapflox/flox and BKO mice were characterized.

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