Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Longitudinal variations in the tprK gene of Treponema pallidum in an Amoy strain-infected rabbit model

PROVIDER: PRJNA943511 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23822626_1.fastq.gz	Fastqsanger.gz
	SRR23822626_2.fastq.gz	Fastqsanger.gz
	SRR23822627_1.fastq.gz	Fastqsanger.gz
	SRR23822627_2.fastq.gz	Fastqsanger.gz
	SRR23822628_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 136

Similar Datasets

Treponema pallidum subsp. pallidum

Project description:Sequencing of tprK in Treponema pallidum subsp. pallidum

| PRJNA589065 | ENA

Gene expression of Treponema pallidum: in vitro vs. in vivo

Project description:Treponema pallidum ssp. pallidum, the causative agent of syphilis, can now be cultured continuously in vitro utilizing a tissue culture system, and the multiplication rates are similar to those obtained in experimental infection of rabbits. In this study, the RNA transcript profiles of the T. pallidum Nichols during in vitro culture and rabbit infection were compared to examine whether gene expression patterns differed in these two environments. To this end, RNA preparations were converted to cDNA and subjected to RNA-seq using high throughput Illumina sequencing; reverse transcriptase quantitative PCR was also performed on selected genes for validation of results. The transcript profiles in the in vivo and in vitro environments were remarkably similar, exhibiting a high degree of concordance overall. However, transcript levels of 94 genes (9%) out of the 1,063 predicted genes in the T. pallidum genome were significantly different during rabbit infection versus in vitro culture, varying by up to 8-fold in the two environments. Genes that exhibited significantly higher transcript levels during rabbit infection included those encoding multiple ribosomal proteins, several prominent membrane proteins, glycolysis-associated enzymes, replication initiator DnaA, rubredoxin, thioredoxin, two putative regulatory proteins, and proteins associated with solute transport. In vitro cultured T. pallidum had higher transcript levels of DNA repair proteins, cofactor synthesis enzymes, and several hypothetical proteins. The overall concordance of the transcript profiles may indicate that these environments are highly similar in terms of their effects on T. pallidum physiology and growth, and may also reflect a relatively low level of transcriptional regulation in this reduced genome organism.

2021-10-12 | GSE185709 | GEO

Project description:rabbit and Treponema pallidum genome sequencing

| PRJNA305961 | ENA

Treponema pallidum ssp. pallidum global proteome analysis (in vitro-cultured treponemes).

Project description:Proteome-wide analysis of the syphilis spirochete, Treponema pallidum ssp. pallidum (Nichols strain). Treponemes were in vitro-cultured.

2023-12-07 | MSV000093603 | MassIVE

Genome-wide microRNAs analysis of peripheral blood mononuclear cells reveals elevated miR-142-3p expression as a potential biomarker for secondary syphilis

Project description:Treponema pallidum subspecies pallidum (T. pallidum) infection induces significant immune responses, resulting in tissue damage. Gene expression plays an essential role in regulating the progression of syphilis infection. However, little is known about the regulatory role of miRNAs in the immune response to T. pallidum infection. Here, we analyze the differential expression of miRNAs in peripheral blood mononuclear cells (PBMCs) between secondary syphilis (SS) patients and healthy controls and study the correlation between miRNAs expression and clinical features with bioinformatics.

2020-08-19 | GSE156421 | GEO

Treponema pallidum ssp. pallidum global proteome analysis (in vivo-cultured treponemes)

Project description:Proteome-wide analysis of the syphilis spirochete, Treponema pallidum ssp. pallidum (Nichols strain). Treponemes were cultured in, and isolated from, New Zealand white rabbits.

2023-05-24 | MSV000092028 | MassIVE

Treponema pallidum SS14 global proteomics

Project description:Raw data files (RAW and mzML) and Scaffold search engine files corresponding to the whole proteome analyses of the syphilis spirochete, Treponema pallidum ssp. pallidum, strain SS14.

| MSV000095818 | MassIVE

Early innate immunity determines outcome of Mycobacterium tuberculosis pulmonary infection in rabbits

Project description:In our rabbit model of pulmonary tuberculosis, infection with Mtb HN878, a hyper-virulent W-Beijing strain, results in progressive cavitary disease. However, infection of rabbit lungs with Mtb CDC1551, a hyper-immunogenic strain is effectively controlled overtime, establishing latent Mtb infection. Using these two Mtb strains, we tested the hypothesis that the initial host response in the lungs within hours of infection determines later outcome. The microarray experiments was performed to identify gene expression changes in the Mtb-HN878 or CDC1551- infected rabbit lungs at 3 hours post infection, compared to uninfected naïve rabbit lungs.

2013-08-17 | GSE49947 | GEO

Lysinoalanine crosslinking is a conserved post-translational modification in the spirochete flagellar hookLysinoalanine crosslinking is a conserved post-translational modification in the spirochete flagellar hook

Project description:In this project, we investigated whether diverse spirochete species contain lysinoalanine cross-linkages between conserved residues in their flagellar hook proteins (FlgE). We examined the following species: Treponema denticola¸ Borreliella burgdorferi, Treponema phagedenis, Treponema pallidum, Brachyspira hyodysenteriae, Leptospira interrogans. For each bacterial species, we examined a variety of different sample types, including recombinantly produced FlgE, polyhooks purified from flik knock-out strains, and wild-type periplasmic filaments. Overall, our data suggests that lysinoalanine crosslinking in a conserved FlgE post-translational modification in spirochetes and is required for the unique motility of these organisms. For more information, please see the following manuscript: https://doi.org/10.1101/2023.06.13.544825.

2024-01-26 | PXD045997 | Pride

Project description:Treponema pallidum strain A10 genome

| PRJEB25146 | ENA