Project description:Reprogramming a non-methylotrophic industrial host, such as Saccharomyces cerevisiae, to a synthetic methylotroph reprents a huge challenge due to the complex regulation in yeast. Through TMC strategy together with ALE strategy, we completed a strict synthetic methylotrophic yeast that could use methanol as the sole carbon source. However, how cells respond to methanol and remodel cellular metabolic network on methanol were not clear. Therefore, genome-scale transcriptional analysis was performed to unravel the cellular reprograming mechanisms underlying the improved growth phenotype.

2024-05-02 | GSE173802 | GEO

Saccharomyces cerevisiae BY4741

Project description:Improving the Z3EV promoter system to create the strongest yeast promoter

| PRJDB18827 | ENA

Saccharomyces cerevisiae BY4741

Project description:Nanopore sequencing improves construction of customized CRISPR-based gene activation libraries

| PRJNA1049995 | ENA

Saccharomyces cerevisiae BY4741

Project description:Genome-wide base editor screen identifies regulators of protein abundance in yeast

| PRJNA732764 | ENA

Saccharomyces cerevisiae isolate:BY4741 | breed:S288C | cultivar:BY4741

Project description:C' AID-GFP library yeast strains Raw sequence reads