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Analysis of nucleus and cytoplasm-specific HNRNPK binding on SINEUP RNA by seCLIP

ABSTRACT: Analysis of nucleus and cytoplasm-specific HNRNPK binding on SINEUP RNA by seCLIP

PROVIDER: PRJNA944232 | ENA |

REPOSITORIES: ENA

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Analysis of nucleus and cytoplasm-specific HNRNPK binding on SINEUP RNA by seCLIP

Project description:To check the subcellular location-specific HNRNPK binding sites on mouse SINEB2 repeat element, we prepared seCLIP (single-end enhanced crosslinking and immunoprecipitation assay) libraries from SINEUP-GFP transfected nuclear and cytoplasmic fractions from HEK293T/17 cells.

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SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assembly [eCLIP-seq]

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Detection of SINEUP RNA modification by Oxford Nanopore direct-RNA sequencing

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hnRNPK knock down in Ewing cell line

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