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Transcriptomic profile of CD8+ circulating tumor-reactive lymphocytes (CTRL) isolated by MHC multimers

ABSTRACT: Transcriptomic profile of CD8+ circulating tumor-reactive lymphocytes (CTRL) isolated by MHC multimers

PROVIDER: PRJNA944679 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23864586_1.fastq.gz	Fastqsanger.gz
	SRR23864586_2.fastq.gz	Fastqsanger.gz
	SRR23864587_1.fastq.gz	Fastqsanger.gz
	SRR23864587_2.fastq.gz	Fastqsanger.gz
	SRR23864588_1.fastq.gz	Fastqsanger.gz

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Transcriptomic profile of CD8+ circulating tumor-reactive lymphocytes (CTRL) isolated by MHC multimers

Project description:Circulating tumor-reactive lymphocytes (CTRL) present in blood circulation at a very low frequency. We efficiently isolated them based on high-performance microfluidics and MHC multimer binding assay. Using an ultra-low input bulk RNAseq workflow, we systematically characterize the transcriptomic profile of tumor-reactive lymphocytes and identified novel biomarkers for multimer-free isolation. Two animal models were studied - B16 melanoma model and CT-26 colon cancer model.

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