Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [TOP2Bcc-seq]

ABSTRACT: Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [TOP2Bcc-seq]

PROVIDER: PRJNA945091 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23873987_1.fastq.gz	Fastqsanger.gz
	SRR23873987_2.fastq.gz	Fastqsanger.gz
	SRR23873988_1.fastq.gz	Fastqsanger.gz
	SRR23873988_2.fastq.gz	Fastqsanger.gz
	SRR23873989_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [fastGRO]

Project description:Topoisomerase IIb (TOP2B) is essential for neural development and function, yet its precise molecular roles remain poorly understood. Here we trapped catalytically engaged TOP2B in DNA cleavage complexes (TOP2Bccs) and mapped their positions genome-wide in cultured mouse cortical neurons. We report that chromosome compartments set the threshold of TOP2B activity within the genome, while specific nucleosome configurations stimulate TOP2B activity within strongly transcribed regions and enhancers. Highly expressed genes that are devoid of usually associated chromatin marks, such as H3K36me3, are deficient in TOP2B activity, indicating that TOP2B may be inefficient at sensing transcription-generated torsional stress directly. Active promoters and the transcription start sites (TSS) with high RNA polymerase II (RNAPII) occupancy show elevated TOP2B ChIP-seq signals but are depleted in TOP2Bccs, indicating that TOP2B is held inactive at active promoters. Surprisingly, TOP2B inhibition with etoposide increased nascent transcription at highly expressed genes and enhancers. While ETP treatment reduced nascent transcription within long genes, these effects were independent of transcript length and instead correlated with the presence of intragenic enhancers. Together these results provide insights into the epigenetic regulation of topoisomerase activity and reveal new roles for TOP2B in neuronal transcriptional regulation.

2024-01-29 | GSE227458 | GEO

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [Re-analysis_TOP2B_mm10]

2024-01-29 | GSE227462 | GEO

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [Re-analγsis_gH2ax_mm10]

2024-01-29 | GSE227461 | GEO

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [RNA-Seq]

2024-01-29 | GSE227459 | GEO

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [TOP2Bcc-seq]

2024-01-29 | GSE227460 | GEO

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [CTCF ChIP-Seq]

2024-01-29 | GSE227457 | GEO

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome

Project description:Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome

| PRJNA945087 | ENA

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [fastGRO]

Project description:Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [fastGRO]

| PRJNA945089 | ENA

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [RNA-Seq]

Project description:Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [RNA-Seq]

| PRJNA945090 | ENA

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [CTCF ChIP-Seq]

Project description:Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome [CTCF ChIP-Seq]

| PRJNA945088 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data