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Rhizospheric soil of codonopsis codonopsis

ABSTRACT: Rhizosphere soil bacteria of Codonopsis codonopsis Genome sequencing

PROVIDER: PRJNA945225 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24032134_1.fastq.gz	Fastqsanger.gz
	SRR24032134_2.fastq.gz	Fastqsanger.gz
	SRR24032135_1.fastq.gz	Fastqsanger.gz
	SRR24032135_2.fastq.gz	Fastqsanger.gz
	SRR24032136_1.fastq.gz	Fastqsanger.gz

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Project description:Arsenic (As) bioavailability in the rice rhizosphere is influenced by many microbial interactions, particularly by metal-transforming functional groups at the root-soil interface. This study was conducted to examine As-transforming microbes and As-speciation in the rice rhizosphere compartments, in response to two different water management practices (continuous and intermittently flooded), established on fields with high to low soil-As concentration. Microbial functional gene composition in the rhizosphere and root-plaque compartments were characterized using the GeoChip 4.0 microarray. Arsenic speciation and concentrations were analyzed in the rhizosphere soil, root-plaque, porewater and grain samples. Results indicated that intermittent flooding significantly altered As-speciation in the rhizosphere, and reduced methyl-As and AsIII concentrations in the pore water, root-plaque and rice grain. Ordination and taxonomic analysis of detected gene-probes indicated that root-plaque and rhizosphere assembled significantly different metal-transforming functional groups. Taxonomic non-redundancy was evident, suggesting that As-reduction, -oxidation and -methylation processes were performed by different microbial groups. As-transformation was coupled to different biogeochemical cycling processes establishing functional non-redundancy of rice-rhizosphere microbiome in response to both rhizosphere compartmentalization and experimental treatments. This study confirmed diverse As-biotransformation at root-soil interface and provided novel insights on their responses to water management, which can be applied for mitigating As-bioavailability and accumulation in rice grains.

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Codonopsis pilosula

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Project description:Codonopsis pilosula, a kind of traditional Chinese medicine, possesses anti-aging effect. However, the anti-aging effect of Codonopsis pilosula on lung remains largely unknow and the exact molecular mechanism of Codonopsis pilosula anti-aging also needs to be further studied. Thus, we investigated the protective effect of Codonopsis pilosula on aging lung and the exact molecular mechanism of Codonopsis pilosula anti-aging. To test this hypothesis, we established the aging mice model, and then used Codonopsis pilosula to intervene. Microarray analysis and bioinformatics methods were used to comprehensively analyze lncRNA-miRNA-mRNA (ceRNA) network. Our results showed that the aging mice model was established. From the microarray analysis, 138 lncRNAs, 128 mRNAs and seven miRNAs significantly changed after aging, whereas, 282 lncRNAs, 283 mRNAs and 19 miRNAs were dysregulated after intervention of Codonopsis pilosula. KEGG pathway analysis was performed to explore the signaling pathways involved. Compared with the ceRNA network in aging mice and after intervention of Codonopsis pilosula, we found three mRNAs (Hif3a, Zbtb16, Plxna2) and one lncRNA (NONMMUT063872) associated with Codonopsis pilosula anti-aging and they were validated by qRT-PCR. Altogether, our results showed that Codonopsis pilosula has the protective effect on aging lung and the ceRNA network plays an important role in Codonopsis pilosula anti-aging.

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