Project description:We used a positron emission tomography (PET) tracer 18F fluorodeoxyglucose ([18F]-FDG) and transcriptomic analysis to detect glucose uptake by cells in the bone marrow micro-environment with an MLL-AF9-induced mouse model. Leukaemic cells had the greatest glucose uptake. To determine whether glucose uptake is driven by intrinsic demand, we applied RNA-seq of sorted leukaemia cells (GFP+) and bone marrow micro-environment myeloid cells (GFP-CD11b+) from the MLL-AF9 transduced mouse model.
Project description:To identify the metabolic transcriptome landscape within leukaemic cells we performed scRNA-Seq on the sorted leukaemia cells (GFP+) cells from MLL-AF9 mice.
Project description:The aim of this study was to investigate the global transcriptional changes induced by high glucose environment in primary osteoblasts in vitro. We isolated bone marrow stromal cells from 4-week-old Sprague-Dawley rats, differentiated them into osteoblasts and exposed them to a short- (one or three days, abbr. HG1d and HG3d) or a long-term (ten days, HG10d) high glucose environment, after which global transcriptional changes were studied using mRNA sequencing. Overall, the cells were differentiated for 10 days and high glucose treatments were started on days 9, 7, and 0 of differentiation.