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Oligoadenylate synthetase-like (OASL) aggravated newcastle disease virus (NDV)-induced necroptosis in glioma cells

ABSTRACT: Oligoadenylate synthetase-like (OASL) aggravated newcastle disease virus (NDV)-induced necroptosis in glioma cells

PROVIDER: PRJNA947071 | ENA |

REPOSITORIES: ENA

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Oligoadenylate synthetase-like (OASL) aggravated newcastle disease virus (NDV)-induced necroptosis in glioma cells

Project description:Newcastle disease virus (NDV) has emerged as an oncolytic agent in several cancers. Previous study has shown that NDV exerts cytolytic activity in glioma, however, the underlying mechanism has not been fully uncovered. Here the cytolytic activity of NDV in glioma and the associated mechanisms have been demonstrated. Infection with NDV inhibits cell proliferation and promotes cell apoptosis in LN229 cells. Further investigation showed that cytoplasmic organelle damage and cytoplasmic vacuolation were observed in LN229 cells after NDV infection. JC-1 staining assay proved that NDV caused cell apoptosis of LN229 cells by inducing mitochondrial dysfunction. We next speculated that NDV caused LN229 cells death through inducing necroptosis, but not ferroptosis, since the Fe2+ level did not alter after NDV infection. Furthermore, the NDV-caused cell death in LN229 cells was blocked by necroptosis inhibitor Nec1. Besides, RNA-seq analysis identified the different expression genes in NDV-infected LN229 cells. OASL, an antiviral gene, has been found to be directly induced by NDV infection. We also found that knockdown of OASL enhanced NDV infection-induced LN229 cells necroptosis. In summary, two aspects about cytolytic activity of NDV in glioma have been demonstrated. NDV presented cytolytic activity in glioma cells through inducing necroptosis. Additionally, targeting OASL may provide new strategy for enhancing necroptosis of glioma cells after NDV infection.

2023-05-01 | GSE227791 | GEO

DEF cells infected with NDV II

Project description:To investigate the role of gene expression during Newcastle disease virus (NDV) infection.The NDV GM strain was used to infect DEF cells with 1moi, while an uninfected group was set up as a control.

2023-04-11 | GSE229335 | GEO

Transposon mutagenesis screening of Newcastle disease virus (NDV)

Project description:Transposon mutagenesis screening of Newcastle disease virus (NDV)

| PRJNA575639 | ENA

Newcastle disease virus activates methylation-related enzymes to reprogram m6A Methylation in infected cells

Project description:To investigate the role of m6A modification during Newcastle disease virus (NDV) infection We then performed gene expression profiling analysis using data obtained from RNA-seq and MeRIP-seq of NDV infected CEF cells and normal cells.

2023-12-31 | GSE220921 | GEO

Expression data of spleens from chickens infected with different Newcastle Disease Viruses

2012-08-15 | GSE40100 | GEO

RNA-Seq Analysis of Spleen from Chicken and Japanese Quail infected with Lentogenic and Velogenic Newcastle Disease Virus

Project description:Newcastle disease (ND) affects a few hundred avian species including chicken, and the clinical outcome of Newcastle disease virus (NDV) infection ranges from mild to severe fatal disease depending on the NDV pathotype and the host species involved. Japanese quails serve as natural reservoirs of NDV and play important role in NDV epidemiology. While infection of chicken with velogenic NDV results in severe often fatal illness, the same infection in Japanese quails is results in in apparent infection. The molecular basis of this contrasting clinical outcomes of NDV infection is not yet known. We compared global gene expression in spleens of chicken and Japanese quails infected with a lentogenic or velogenic NDVs. We found contrasting regulation of key genes associated with NF-κB pathway and T-cell activation between chicken and Japanese quails. Our data suggests association of NDV resistance in Japanese quails to activation of NF-κB pathway and T cell proliferation.

2018-12-31 | GSE98296 | GEO

Transposon mutagenesis screening of Newcastle disease virus (NDV)

Project description:We report the genetic plasticity of Newcastle disease virus. We introduced insertional mutation in the virus genome and checked fitness by comparing distribution of mutants in passage 1 and passage 2.

2019-10-04 | GSE138366 | GEO

Transcriptome Analysis of Bladder Cancer Cell Persistently Infected by Newcastle disease virus (NDV)

Project description:This project seeks to determine if there is a difference in expression between bladder cancer cells vs NDV-resistant bladder cancer cells.This result provides insight of the orchestrated transcriptomic changes that occur in the development and maintenance of NDV persistency of infection, which can be an useful resource for future downstream analysis of the identified targets.

2020-12-28 | GSE163881 | GEO

Expression data of spleens from chickens infected with different Newcastle Disease Viruses

Project description:Genotype VIId NDV is characterized by severe tissue damage in chicken lymphoid organs compared to other virulent strains. However, biological basis of this unusual pathological phenotype is unknown. Host response is associated with pathogenicity of Newcastle Disease Virus (NDV). We aim to determine the contribution of host response to the severe tissue destruction in the lymphoid system caused by genotype VIId NDV. We used microarray analysis to evaluate the global transcriptional response in the spleen of chickens infected with genotype VIId NDV strain JS5/05 and genotype IV NDV Herts/33. Chickens were inoculated with JS5/05 or Herts/33 or mock-infected. At day 2 post infection, spleens were isolated from three chickens per group for RNA extraction and hybridization on Affymetrix microarrays. Samples were named as follows: JS5/05 (I4_1_NS,I4_2_NS,I4_3_NS), Herts/33 (H1_NS,H2_NS, H3_NS), control (C1_NS, C2_NS, C3_NS).

2012-08-14 | E-GEOD-40100 | biostudies-arrayexpress

Transcriptome profiling data of urothelial cell carcinoma (UCC) persistently infected with oncolytic Newcastle disease virus

Project description:Newcastle disease virus (NDV) is an avian virus that selectively replicates and kills many different types of cancer cells and is being developed for cancer treatment. Our aim was to establish persistent infection in EJ28 and TCCSUP bladder cancer cells and identify the dysregulated genes and disrupted molecular pathways associated with persistent infection.

2019-11-25 | GSE140902 | GEO

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