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Lactic acid production bioreactor

ABSTRACT: Lactic acid production bioreactor Raw sequence reads

PROVIDER: PRJNA947195 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23962338.fastq.gz	Fastqsanger.gz
	SRR23962339.fastq.gz	Fastqsanger.gz
	SRR23962340.fastq.gz	Fastqsanger.gz
	SRR23962341.fastq.gz	Fastqsanger.gz
	SRR23962342.fastq.gz	Fastqsanger.gz

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Conferring Flocculation Phenotype to Pichia pastoris Improves D-lactic Acid Production and Tolerance

Project description:D-lactic acid is a three-carbon organic acid with a chiral structure and can improve the thermostability of polylactic acid. Microorganisms such as the methylotrophic yeast Pichia pastoris, which lack the natural ability to produce or accumulate high amounts of D-lactic acid, have been engineered to produce it in high titers. However, tolerance to D-lactic acid remains a challenge. In this study, we demonstrate that cell flocculation improves tolerance to D-lactic acid and leads to increased D-lactic acid production in Pichia pastoris. By incorporating a flocculation gene from Saccharomyces cerevisiae (ScFLO1) into P. pastoris KM71, we created a strain (KM71-ScFlo1) that demonstrated up to a 1.6-fold improvement in specific growth rate at high D-lactic acid concentrations. Furthermore, integrating a D-lactate dehydrogenase gene from Leuconostoc pseudomesenteroides (LpDLDH) into KM71-ScFlo1 resulted in an engineered strain (KM71-ScFlo1-LpDLDH) that can produce D-lactic acid at a titer of 5.12  0.35 g/L in 48 hours , a 2.6-fold improvement over the control strain lacking ScFLO1 expression. Transcriptomics analysis of this strain provided insights into the mechanism of increased tolerance to D-lactic acid including the upregulations of genes involved in lactate transport and iron metabolism. Overall, our work represents an advancement in the efficient microbial production of D-lactic acid by manipulating yeast flocculation.

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