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Effect of IL-12+IL-18 or anti-NK1.1 stimulation on splenic NK cells [mNK_Activation_RNA-seq]

ABSTRACT: Effect of IL-12+IL-18 or anti-NK1.1 stimulation on splenic NK cells [mNK_Activation_RNA-seq]

PROVIDER: PRJNA947204 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23933522.fastq.gz	Fastqsanger.gz
	SRR23933523.fastq.gz	Fastqsanger.gz
	SRR23933524.fastq.gz	Fastqsanger.gz
	SRR23933525.fastq.gz	Fastqsanger.gz
	SRR23933526.fastq.gz	Fastqsanger.gz

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Effect of IL-12+IL-18 or anti-NK1.1 stimulation on splenic NK cells [mNK_Activation_RNA-seq]

Project description:We stimulated freshly enriched splenic NK cells with either IL-12+IL-18 (1ng/ml each) or plate-bound anti-NK1.1. NKs from several C57Bl/6 mice were pooled. We then performed gene expression profiling analysis using data obtained from RNA-seq at two time points: 3 or 6 hours.

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Effect of IL-15 culture +/- anti-NK1.1 stimulation on splenic NK cells [mNK_IL-15_RNA-seq]

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Project description:We analyzed gene expression profiles of IL-18 generated murine NK cells in comparison to unstimulated, freshly isolated splenic NK cells. We identified a set of 1414 Affymetrix probe sets showing significant misregulation (Welch's T-test, p<0.05; Benjamini-Hochberg FDR corrected). IL-18 generated as well as unstimulated NK cells were isolated in three independent preparations and used for RNA extraction and hybridization on Affymetrix microarrays.

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