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CUT-TAG for NB4 cell line with either IgG or LDB1 antibody

ABSTRACT: CUT-TAG for NB4 cell line with either IgG or LDB1 antibody

PROVIDER: PRJNA947227 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23933137_1.fastq.gz	Fastqsanger.gz
	SRR23933137_2.fastq.gz	Fastqsanger.gz
	SRR23933138_1.fastq.gz	Fastqsanger.gz
	SRR23933138_2.fastq.gz	Fastqsanger.gz

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CUT-TAG for NB4 cell line with either IgG or LDB1 antibody

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CUT-TAG for NB4 cell line with either no treatment or LDB1 overexpression

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Project description:Saliva based diagnostics is a rapidly evolving field due to the large potential of saliva and the simple sample collection. A systematic comparison of IgG antibody profiles in saliva and plasma is currently lacking in scientific literature. Our hypothesis is that IgG profiles are equal in blood and saliva. By showing the equality of the profiles and relative IgG antigenic reactivities towards proteins and peptides we provide evidence that plasma IgG reactivities can be inferred from saliva IgG reactivities. IgG antibodies were isolated from human saliva and plasma samples. The reactivities of IgG isolates were analysed on peptide microarrays displaying linear epitopes of EBV (EBNA1 protein) and HBV (Large envelope protein) virus. Peptide arrays were printed by JPT Peptide Technologies (Berlin, Germany). We show high similarity of saliva and plasma IgG profiles on these two platforms and argue for generalisation from this subset to the whole immunological IgG antibody profile.

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