Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

IL-15 priming alters IFN-g regulation in murine NK cells.

ABSTRACT: IL-15 priming alters IFN-g regulation in murine NK cells.

PROVIDER: PRJNA947243 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23933508.fastq.gz	Fastqsanger.gz
	SRR23933509.fastq.gz	Fastqsanger.gz
	SRR23933510.fastq.gz	Fastqsanger.gz
	SRR23933511.fastq.gz	Fastqsanger.gz
	SRR23933512.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 60

Similar Datasets

IL-15 priming alters IFN-g regulation in murine NK cells.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-08-01 | GSE227894 | GEO

Short-term hypoxia synergizes with interleukin 15 priming in driving glycolytic gene transcription and supports human natural killer cell activities.

Project description:Natural killer (NK) cells induce apoptosis in infected and transformed cells and produce immunoregulatory cytokines. At this, NK cells operate in inflammatory and tumor environments low in oxygen (hypoxic) and with immunosuppressive properties. In vitro studies of NK cells are, however, commonly performed in ambient air (normoxia). We evaluated the immediate impact of short-term hypoxia on various intrinsic activities of resting and IL-15 primed NK cells and determined underlying transcriptional pathway regulations using a 2 × 2 factorial design. Hypoxia stimulated migration through extracellular matrix, supported secretion of specific chemokines, and shifted amounts of susceptible leukemia target cells toward late apoptosis. IL-15 was a powerful inducer of anabolic gene transcription. Associations of hypoxia with HIF-1 and glycolytic pathways were dependent on priming which represented the largest contrasts observed. Regulatory patterns and RT-PCR validated synergistic hypoxia-IL-15 interactions in upregulation of key glycolytic enzymes suggest an important function for anaerobic glycolysis in priming, underpinning the emerging role of glycolytic switching in driving NK cell function. Adaption to short-term hypoxia appeared however transitory. We conclude that control of oxygen in vitro can promote NK cell features desirable for adoptive transfer therapy.

2016-05-09 | GSE70214 | GEO

Transcriptome analysis of TIML- and CIML-NK cells

Project description:NK cells may acquire under certain conditions features of adaptive immune cells. As the functional role of memory NK cells in cancer has so far remained elusive, we reasoned whether tumor-priming itself might promote memory NK cell generation. We provide substantial evidence that independent from pro-inflammatory stimulation, tumor-induced memory-like (TIML) NK cells exhibit a heightened, tumor-restricted cytotoxicity which is dependent on a higher/faster perforin but not IFN-Î³ release. Comparative transcriptome analysis reveals that gene expression patterns differ between TIML- and Cytokine-induced memory-like (CIML)-NK cells.

2016-04-15 | E-MTAB-4606 | biostudies-arrayexpress

Phosphoproteomics of Natural Killer (NK) Cells Stimulated with Fibrin and IL-15

Project description:Phosphoproteomic analysis of fibrin or IL-15 treated NK cells with timsTOF HT

2024-06-26 | PXD049692 | Pride

Transcriptome Sequencing of Neo-2/15 stimulated CAR-NK cells

Project description:We performed transcriptome sequencing on Neo-2/15 stimulated CAR NK cells,to shed light on the function and phenotype changes of CAR-NK cells stimulated by IL-2 and Neo-2/15.

2023-12-31 | GSE243530 | GEO

RNA squencing of human NK cells activated with IL-15 or IL-12, IL-15, and IL-18

Project description:NK cells are an emerging cancer cellular therapy and potent mediators of anti-tumor immunity. Cytokine-induced memory-like (ML) NK cellular therapy is safe and induces remissions in acute myeloid leukemia (AML) patients. However, the dynamic molecular changes that occur after memory-like differentiation in vitro are unclear. Here, control or ML NK cells purified from normal donor PBMC were generated in vitro. Briefly, RosetteSep-purified NK cells were incubated in IL-12, IL-15, and IL-18, or low-dose IL-15 as a control for 16-18 hours. Control or cytokine-activated NK cells were washed three times and cultured for 6 days in low-dose IL-15, which is required for NK cell survival. After 6 days, RNA was isolated from control and memory-like (ML) NK cells (IL12/15/18 activation) and RNA-sequencing performed. Because the transcription factor GATA-3 was increased specifically in ML NK cells, we hypothesized ML NK cells would exhibit a GATA-3 gene signature compared to control NK cells. Indeed, using GSEA, a significant gene signature was associated with ML NK cell differentiation. These data support the role for GATA-3 in regulating the ML NK cell molecular program.

2020-08-25 | GSE154694 | GEO

Runx3 function in splenic NK cells (IL-2 or IL-15)

Project description:NK cells are innate immune cells that recognize and kill foreign, virally-infected and tumor cells without the need for prior immunization. NK expansion following viral infection is IL-2 or IL-15-dependent. To identify Runx3 responsive genes, NK cells were isolated from spleen of WT and Runx3-/- mice . Ten samples (5 WT and 5 Runx3-/-) of freshly isolated NK cells were separately obtained from individual mice. Cells were cultured for 7 days with IL-2 or IL-15.

2013-11-18 | E-GEOD-50122 | biostudies-arrayexpress

mRNA expression profiles of resting and IL-15 activated murine NK cells

Project description:Murine NK cells were compared at rest and following 24 hours of IL-15 stimulation for their mRNA expression profiles on the Affymetrix MOE430_2 microarray platform. Additional comparators included resting bulk splenocytes. Keywords: mRNA, mouse, NK cell, IL-15

2007-05-15 | GSE7764 | GEO

Genome-wide CRISPR screen reveals cancer cell resistance to NK cells induced by NK-derived IFN-gamma

Project description:The anti-leukemia activity of NK cells helps to prevent relapse during hematopoietic stem cell transplantation in leukemia patients. However, the factors that determine sensitivity or resistance of leukemia cells in the context of NK-mediated cytotoxicity are not well established. Here we performed a genome-wide CRIPSR screen in the human chronic-myelogenous-leukemia (CML) cell line K562 to identify genes that regulate vulnerability of leukemia cells to killing by primary human NK cells. Distribution of guide RNAs (gRNAs) in K562 cells that survived co-incubation with NK cells showed that loss of NCR3LG1, which encodes the ligand of the natural cytotoxicity receptor NKp30, protected K562 cells from killing. In contrast, loss of genes that regulate pathways for antigen-presentation and interferon-gamma-signaling increased the vulnerability of K562 cells. Addition of IFN-gamma neutralizing antibody increased the susceptibility of K562 cells to NK-mediated killing. Upregulation of MHC class I on K562 cells after co-incubation with NK cells was dependent on IFNGR2. Analysis of RNA-seq data from The Cancer Genome Atlas (TCGA) showed that low IFNGR2 expression in cancer tissues associated with improved overall survival in acute myeloid leukemia (AML) and Kidney Renal Clear Cell Carcinoma (KIRC) patients. Our results showing that upregulation of MHC class I by NK-derived IFN-gamma leads to resistance to NK cytotoxicity suggest that targeting IFN-gamma responses might be a promising approach to enhance NK cell anti-cancer efficacy.

2019-10-24 | GSE139313 | GEO

Runx3 function in splenic NK cells (IL-2 or IL-15)

2013-11-18 | GSE50122 | GEO