Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

4C-Seq Drosophila Imaginal Disc HP6/Umbrea

ABSTRACT: 4C-Seq Drosophila Imaginal Disc HP6/Umbrea

PROVIDER: PRJNA948431 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Wing imaginal disc spatial expression

Project description:Wing imaginal discs were dissected to generate body wall and wing/hinge fragments. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix Drosophila Genechip 1 arrays. Comparisons between the sample groups allow the identification of genes with localized expression patterns. Keywords = Drosophila, wing disc, spatially restricted transcripts Keywords: repeat sample

2003-01-08 | GSE93 | GEO

Expression data from transdetermined Drosophila eye antennal imaginal disc by winged eye overexpression.

Project description:Drosophila imaginal disc cells exhibit a remarkable ability to switch cell fates under various perturbations, a phenomenon known as transdetermination (TD). The winged eye (wge) gene induces eye-to-wing TD by its overexpression in eye imaginal discs using eye specific Gal4 driver (eyeless-Gal4). Gene network controlling this process, however, is largely unclear. Additionally, we identified that heterochromatin-related histone methyltransferase Su(var)3-9 is essential for wge-mediated TD. We used microarray to detail the global gene network underlying wge-mediated eye-to-wing TD, and the involvement of Su(var)3-9 in the gene network.

2017-12-31 | GSE87622 | GEO

Eye imaginal disc development

Project description:Coordinating cell proliferation and differentiation is essential during organogenesis. In Drosophila, the photoreceptor, pigment and support cells of the eye are specified in an orchestrated wave as the morphogenetic furrow passes across the eye imaginal disc. Cells anterior of the furrow are uncommitted to cell type and remain mitotically active, while most cells in the furrow arrest at G1 and adopt specified ommatidial fates. We used microarray expression analysis to monitor changes in transcription at the furrow and identified genes whose expression correlates with either proliferation or fate specification.

2008-09-23 | GSE12851 | GEO

4C-Seq Drosophila Imaginal Disc HP6/Umbrea

Project description:Chromosomal conformation capture in Drosophila melanogaster with a viewpoint of the HP6/Umbrea locus

2024-07-16 | GSE228170 | GEO

Expression data from transdetermined Drosophila eye antennal imaginal disc by vestigial and simjang overexpression.

Project description:Drosophila imaginal disc cells exhibit a remarkable ability to switch cell fates under various perturbations, a phenomenon known as transdetermination (TD). The process of cell reprogramming seems to be regulated in stochastic manners. Ectopic expression of the master transcription factor of wing identity, vestigial, in eye progenitor cells induces eye-to-wing TD, but the frequency of the TD is low. We found that the overexpression of simjang gene dramatically enhanced eye-to-wing TD frequency. Gene network controlling this process, however, is largely unclear. We used microarray to detail the global gene network underlying simj-mediated facilitation of eye-to-wing TD.

2020-12-31 | GSE125425 | GEO

Transcriptional changes associated with endoplasmic reticulum stress in the eye imaginal disc of Drosophila melanogaster

Project description:We obtained transcriptional profiles of third instar eye imaginal disc nuclei with or without endoplasmic reticulum stress using next generation sequencing (NGS). We employed an isolation of nuclei tagged in specific cell type (INTACT) protocol (Ma and Weake, J. of Vis. Exp., 2014) to label nuclear membranes of GMR-GAL4-expressing cells with EGFP. The experimental group also expressed a mutant allele of the membrane visual protein rhodopsin (Rh-1(G69D)), which is known to cause endoplasmic reticulum stress and initiate Unfolded Protein Response (UPR) signaling. The goal of this study was to characterize the transcriptional changes associated with endoplasmic reticulum stress responses in a commonly used platform, the eye imaginal disc.

2021-09-20 | GSE150058 | GEO

Chromatine immunoprecipation of atonal binding sites in Drosophila L3 eye imaginal discs

Project description:Atonal is a proneural transcription factor expressed in the Drosophila eye imaginal discs. To characterize the putative targes of Atonal in the eye discs we have used an endogenously GFP-tagged version of atonal to immunoprecipate disc samples with anti-GFP antibodies

2019-10-09 | GSE138553 | GEO

Transcriptomic analysis of Drosophila leg disc associated myoblasts

Project description:Adult leg muscle precursors are associated to leg imaginal disc, here we provide bulk RNA sequecing of FACS sorted myoblasts from dissected imaginal leg discs at beginning of pupation.

2024-06-28 | GSE245192 | GEO

Drosophila Imaginal disc growth factor 2 promotes cell growth in vitro independently of insulin and is involved in innate immune response in vivo

Project description:Drosophila imaginal disc growth factors (IDGFs) comprise a small protein family of six members belonging to chitinase-like proteins (CLPs), which bind to, but do not cleave chitin or similar carbohydrates. IDGF2 is the prototypical member with known structure and reported to induce the proliferation of imaginal disc cells Cl.8+ in vitro. We characterized the effects of recombinant IDGF2 on tissue culture cells in vitro. We show that it is involved in cell protection from serum deprivation, as well as from the toxic effects of some xenobiotics and metabolites, when the cells are cultivated in serum-free medium conditions. Our results revealed that IDGF2 does not activate insulin pathway. Microarray-based gene expression analysis identified several IDGF2-dependent genes, including genes implicated in innate immune response, Wnt signaling and genes involved in the response to xenobiotics. Consistently, we observed that IDGF2 can be induced in vivo by aseptic or septic injury and high concentration of IDGF2 was detected in garland and pericardial nephrocytes. Our results suggest that IDGF2 is an important and abundant component of Drosophila hemolymph, which shows cytoprotective effects on insect cells in vitro and works as a modulator of multiple signaling pathways involved in morphogenesis, homeostasis and activation of innate immune response.

2016-06-27 | E-MTAB-5007 | biostudies-arrayexpress

Identification of putative encoding smORF genes in drosophila imaginal discs

Project description:We aim to identify the genes that encode for putatives smORF peptides in imaginal leg disc at larval and pupal stages

2023-10-09 | GSE225561 | GEO