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Single cell RNAseq of co-cultured mouse iPSC-derived lung mesenchyme and mouse iPSC-derived lung epithelial progenitors in distal and proximal differentiation media

ABSTRACT: Single cell RNAseq of co-cultured mouse iPSC-derived lung mesenchyme and mouse iPSC-derived lung epithelial progenitors in distal and proximal differentiation media

PROVIDER: PRJNA949245 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23983649.fastq.gz	Fastqsanger.gz
	SRR23983650.fastq.gz	Fastqsanger.gz
	SRR23983651.fastq.gz	Fastqsanger.gz
	SRR23983652.fastq.gz	Fastqsanger.gz
	SRR23983653.fastq.gz	Fastqsanger.gz

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Single cell RNAseq of co-cultured mouse iPSC-derived lung mesenchyme and mouse iPSC-derived lung epithelial progenitors in distal and proximal differentiation media

Project description:Co-cultures of lung epithelium and mesenchyme are useful tools to study epithelial-mesenchymal crosstalk in lung development and disease. However, many previous attempts to generate such co-cultures have yielded poor juxtaposition between the epithelial and the mesenchymal lineage. In addition, induced pluripotent stem cell (iPSC)-derived co-cultures often contain generic mesenchyme that is not necessarily lung-specific. We sought to establish co-cultures of purified mouse iPSC-derived lung-specific mesenchyme and iPSC-derived lung epithelial progenitors. We used a mouse iPSC line carrying a lung mesenchyme-specific reporter/tracer (Tbx4-LERGFP) to generate lung mesenchymal progenitors by directed differentiation via a lateral plate mesodermal progenitor state (induced lung mesenchyme, iLM). In parallel we differentiated a mouse embryonic stem (ES) cell line carrying a Nkx2-1mCherry reporter into lung epithelial progenitor cells using our established directed differentiation protocol. We then combined the purified lung epithelial and mesenchymal progenitor cells and co-cultured them in distal or proximal differentiation media for 1 week on Matrigel. We find that cells self-organize into complex 3-dimensional organoids with closely juxtaposed epithelial and mesenchymal cells. Furthermore, co-culture affects the molecular phenotype of both lineages. Our iPSC-derived co-culture model can provide an inexhaustible source of cells for studying lung development, modeling diseases, and developing therapeutics.

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