Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison the transcriptional changes of HEK293T cells transfected with synthesized vcDNA

ABSTRACT: Comparison the transcriptional changes of HEK293T cells transfected with synthesized vcDNA

PROVIDER: PRJNA949524 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23987733_1.fastq.gz	Fastqsanger.gz
	SRR23987733_2.fastq.gz	Fastqsanger.gz
	SRR23987734_1.fastq.gz	Fastqsanger.gz
	SRR23987734_2.fastq.gz	Fastqsanger.gz
	SRR23987735_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

Comparison the transcriptional changes of HEK293T cells transfected with synthesized vcDNA

Project description:Extrachromosomal circular DNA (eccDNA) is generated from chromosomal templates to play multiple biological roles in different types of cells. However, it is unknown whether viral infection could cause cells to generate viral non-genomic circular DNA (vcDNA). Here, we demonstrated for the first time that Varicella zoster virus (VZV)-infected cells and tissues generate an abundance of vcDNA. We developed a Cre/loxP-mediated in vitro vcDNA/eccDNA synthesizing method. We transfected the synthesized vcDNA into HEK293T cells, RNA-seq analysis indicated that VZV vcDNA 76509|75569 effectively mitigated the innate immune response that was stimulated by linear VZV 75569-76509 DNA.

2025-03-28 | GSE228350 | GEO

HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [miRNA]

Project description:Purpose: Comparison of RNA-sequencing datasets obtained from exosomes of Nef-transfected and Mock-transfected HEK293T cells Methods: Assessment of RNA content of exosomes produced by Nef-transfected HEK293T cells and and Mock-transfected HEK293T cells Results: Differences in a set of microRNAs Conclusions: Nef-transfection induces changes in the microRNA content of exosomes

2018-11-18 | GSE122656 | GEO

HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [mRNA]

Project description:Purpose: Comparison of RNA-sequencing datasets obtained from exosomes of Nef-transfected and Mock-transfected HEK293T cells. Methods: Assessment of RNA content of exosomes produced by Nef-transfected HEK293T cells and and Mock-transfected HEK293T cells. Results: Differences in a set of mRNAs. Conclusions: Nef-transfection might induces changes in the mRNA content of exosomes.

2018-11-18 | GSE122655 | GEO

HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture

Project description:HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture

| PRJNA505882 | ENA

HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [mRNA]

Project description:HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [mRNA]

| PRJNA505883 | ENA

HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [miRNA]

Project description:HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [miRNA]

| PRJNA505884 | ENA

Detect-seq on transfected HEK293T cells [Detect-seq data]

Project description:We used a new technology, named Detect-seq, to perform genome sequencing on transfected HEK293T cells to see DdCBEs' off-target mutations. Besides, targeted-amplicon sequencing and ATAC-seq data were applied to validate the results of Detect-seq.

2022-04-22 | GSE173859 | GEO

Detect-seq on transfected HEK293T cells [Targeted deep sequencing data]

2022-06-01 | GSE176089 | GEO

Mapping PRDM9 binding and its effects in transfected HEK293T cells

Project description:We report ChIP-seq, RNA-seq, and ATAC-seq results from HEK293T cells transfected with various versions of the meiotic recombination protein PRDM9, in order to learn about its DNA-binding properties, its effects on gene expression and chromatin organization, and which epigenetic factors influence binding and recombination outcomes.

2017-05-31 | GSE99407 | GEO

LILRA3-GFP and GFP only Transfected HEK293T cells LC-MSMS

Project description:The leukocyte immunoglobulin-like receptor A3 (LILRA3) is a soluble protein primarily expressed by peripheral blood monocytes and is abundant in sera of healthy donors. Extracellular LILRA3 is anti-inflammatory and displays neuro-regenerative functions in vitro. However, its intracellular expression, distribution and function(s) remain unknown. Using a combination of high-resolution confocal and super-resolution microscopy, we identified intracellular expression of native LILRA3 in the nucleus of peripheral blood monocytes and in vitro derived macrophages. This unexpected nuclear localisation of LILRA3 was confirmed in LILRA3-GFP transfected HEK293T cells. Western blot of proteins fractionated from primary macrophages and the transfected HEK293T cells confirmed nuclear localisation of the native and expressed LILRA3 proteins. Interestingly, most of the LILRA3 in the nucleus was in a monomeric form like the biologically active secreted protein, while those in the other cellular compartments were mixed monomeric, dimeric, and oligomeric forms. The predominant presence of monomeric LILRA3 in the nucleus was independently corroborated in transfected live HEK293T cells using number and molecular brightness (N&B) analysis method. Immunoprecipitation and Mass spectrometric peptide sequencing studies revealed that nuclear LILRA3 co-immunoprecipitated with several nuclear proteins involved in host protein synthesis machinery via direct interaction to a key multifunctional RNA binding protein, the Ewing sarcoma breakpoint region 1 protein (EWS).

2021-04-08 | PXD024602 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data