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Chromatin remodeling in HIF-1a deficient B cell during IgA class switching [ChIP-seq]

ABSTRACT: Chromatin remodeling in HIF-1a deficient B cell during IgA class switching [ChIP-seq]

PROVIDER: PRJNA949562 | ENA |

REPOSITORIES: ENA

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Chromatin remodeling in HIF-1a deficient B cell during IgA class switching [ChIP-seq]

Project description:The interconnection between metabolic programming and chromatin remodeling during IgA class switching suggest that HIF-1a-dependent glycolytic flux may control the epigenetic modification at α class switching region (Sα) in the Igh locus. To test our hypothesis, histone acetylation was analyzed by chromatin immunoprecipitation sequencing (ChIP-seq) (H3K9ac and H3K27ac) in Hif-1a deficient and control B cell during IgA class switching. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K27ac and H3K9ac in B cell from WT and HIF-1a deficient mice.

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Effect of depletion of HIF-1a on gene expression during B cell IgA class switching [RNA-seq]

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