Project description:The experiment compared flounder from the North Sea and the Baltic sea and their reactions on being exposed to water of different salinities
Project description:<p>Four species of phytoplankton representing important bloom-forming species from three globally important phyla (Bacillariophyta, Haptophyta, and Ochrophyte) were cultured in this study. These species include the cosmopolitan diatom <em>Chaetoceros affinis</em> CCMP159 (isolated from Great South Bay, NY, USA, 1958), the haptophytes<em> Chrysochromulina polylepis </em>CCMP1757 (isolated from the North Sea 1988) and <em>Gephyrocapsa oceanica</em> RCC1303 (isolated from Arachon Bay, France, Jan 1999), and the raphidophyte <em>Heterosigma akashiwo </em>strain CCMP 2393 (isolated from Rehoboth Bay, Delaware, USA). Cultures were grown under three conditions: nitrogen-stress, phosphorus-stress, and replete conditions. Intracellular metabolites were extracted from cultures and analyzed with targeted and untargeted mass spectrometry-based metabolomics methods.</p>
Project description:Array CGH analysis of Helicobacter pylori strains isolated from a North American cohort of symptomatic pediatric patients. Keywords: genotyping_design
Project description:The available energy and carbon sources for prokaryotes in the deep ocean remain still largely enigmatic. Reduced sulfur compounds, such as thiosulfate, are a potential energy source for both auto- and heterotrophic marine prokaryotes. Shipboard experiments performed in the North Atlantic using Labrador Sea Water (~2000 m depth) amended with thiosulfate led to an enhanced prokaryotic dissolved inorganic carbon (DIC) fixation.
Project description:The dataset represents the proteome analysis of six sampling dates during the phytoplankton bloom at the island of Helgoland in the North Sea at the long term research station ‘Kabeltonne’ (54° 11' 17.88'' N, 7° 54' 0'' E) in 2016.
Project description:Sea urchins are emblematic marine animals with a rich fossil record and represent instrumental models for developmental biology. As echinoderms, sea urchins display several characteristics that set them apart from other deuterostomes such as their highly regulative embryonic development and their unique pentaradial adult body plan. To determine whether these characteristics are linked to particular genomic rearrangement or gene regulatory rewiring, we introduce a chromosome-scale genome assembly for sea urchin Paracentrotus lividus as well as extensive transcriptomic and epigenetic profiling during its embryonic development. We found that sea urchins show opposite modalities of genome evolution as compared to those of vertebrates: they retained ancestral chromosomal linkages that otherwise underwent mixing in vertebrates, while their intrachromosomal gene order has evolved much faster between sea urchin species that split 60 Myr ago than it did in vertebrates. We further assessed the conservation of the cis-regulatory program between sea urchins and chordates and identified conserved modules despite the developmental and body plan differences. We documented regulatory events underlying processes like zygotic genome activation and transition to larval stage in sea urchins. We also identified a burst of gene duplication in the echinoid lineage and showed that some of these expanded genes are involved in organismal novelties, such as Aristotle's lantern, tube feet, or in the specification of lineages through for instance the pmar1 and pop genes. Altogether, our results suggest that gene regulatory networks controlling development can be conserved despite extensive gene order rearrangement.
Project description:The dataset represents the proteome analysis of 15 sampling dates during the phytoplankton bloom in the Helgoland Roads in the North Sea at the long-term research station ‘Kabeltonne’ (54°11'N 7°54'E, DEIMS.ID https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1) in 2020.
