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BuTT-Seq: a new method for facile profiling of transcription

ABSTRACT: BuTT-Seq: a new method for facile profiling of transcription

PROVIDER: PRJNA950415 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24030027_1.fastq.gz	Fastqsanger.gz
	SRR24030027_2.fastq.gz	Fastqsanger.gz
	SRR24030028_1.fastq.gz	Fastqsanger.gz
	SRR24030028_2.fastq.gz	Fastqsanger.gz
	SRR24030029_1.fastq.gz	Fastqsanger.gz

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BuTT-Seq: a new method for facile profiling of transcription

Project description:A wide range of sequencing methods have been developed to assess nascent RNA transcription and resolve the single-nucleotide position of RNA polymerase genome-wide. These techniques are often burdened with high input material requirements and lengthy protocols. We leveraged the template-switching properties of thermostable group II intron reverse transcriptase (TGIRT) and developed BuTT-Seq (BUlk analysis of nascent Transcript Termini sequencing), which can produce libraries from purified nascent RNA in 6 hours and from as few as 10,000 cells – an improvement of at least 10-fold over existing techniques. BuTT-Seq shows that inhibition of the superelongation complex (SEC) causes promoter-proximal pausing to move upstream in a fashion correlated with subnucleosomal fragments. To address transcriptional regulation in a tissue, BuTT-Seq was used to measure the circadian regulation of transcription from fly heads. All the results indicate that BuTT-Seq is a simple and powerful technique to analyze transcription at a high level of resolution.

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