Triticum aestivum
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ABSTRACT:
PROVIDER: PRJNA951279 | ENA |
REPOSITORIES: ENA
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Project description:To better understand the regulatory mechanisms of water stress response in wheat, the transcript profiles in roots of two wheat genotypes, namely, drought tolerant 'Luohan No.2' (LH) and drought susceptible 'Chinese Spring' (CS) under water-stress were comparatively analyzed by using the Affymetrix wheat GeneChip®. A total of 3831 transcripts displayed 2-fold or more expression changes, 1593 transcripts were induced compared with 2238 transcripts were repressed, in LH under water-stress; Relatively fewer transcripts were drought responsive in CS, 1404 transcripts were induced and 1493 were repressed. Comparatively, 569 transcripts were commonly induced and 424 transcripts commonly repressed in LH and CS under water-stress. 689 transcripts (757 probe sets) identified from LH and 537 transcripts (575 probe sets) from CS were annotated and classified into 10 functional categories, and 74 transcripts derived from 80 probe sets displayed the change ratios no less than 16 in LH or CS. Several kinds of candidate genes were differentially expressed between the LH and CS, which could be responsible for the difference in drought tolerance of the two genotypes.
2012-01-01 | GSE30872 | GEO
Project description:To better understand the regulatory mechanisms of water stress response in wheat, the transcript profiles in roots of two wheat genotypes, namely, drought tolerant 'Luohan No.2' (LH) and drought susceptible 'Chinese Spring' (CS) under water-stress were comparatively analyzed by using the Affymetrix wheat GeneChip®. A total of 3831 transcripts displayed 2-fold or more expression changes, 1593 transcripts were induced compared with 2238 transcripts were repressed, in LH under water-stress; Relatively fewer transcripts were drought responsive in CS, 1404 transcripts were induced and 1493 were repressed. Comparatively, 569 transcripts were commonly induced and 424 transcripts commonly repressed in LH and CS under water-stress. 689 transcripts (757 probe sets) identified from LH and 537 transcripts (575 probe sets) from CS were annotated and classified into 10 functional categories, and 74 transcripts derived from 80 probe sets displayed the change ratios no less than 16 in LH or CS. Several kinds of candidate genes were differentially expressed between the LH and CS, which could be responsible for the difference in drought tolerance of the two genotypes. Two common wheat (Triticum aestivum L.) cultivars, Luohan No.2 (LH) and Chinese Spring (CS), were used for this study. Seedlings at the two leaf stage were stressed by cultured in PEG solutions for 6h, and some other seedlings were cultured in tap water as control. Root samples of LH and CS at 6h after the stress treatment and untreated control were prepared for microarray analysis.
2012-01-01 | E-GEOD-30872 | biostudies-arrayexpress
Project description:Based on EST-based in silico gene expression analysis a 15k oligonucleotid microarray has been developped in order to monitor environmental stress-dependent gene expression changes in the wheat caryopsis. Using this array, the effect of water withdrawal, with and and without additional heat stress, during the first five days of grain development (0-5 DAA) has been investigated on two wheat cultivars differing in their drought sensitivity. The combined effect of heat and drought (DH) on gene expression was much significant (8-10% of the investigated genes changed >2-fold) in contrast to drought alone (1.5%). Drought and heat stress resulted in the co-ordinated change of the expression of storage proteins, some enzymes involved in sugar/starch metabolism, cell division-related and histone proteins, certain transcription factors, heat shock proteins, proteases and aquaporins. The potential link between the observed gene expression changes and the parallel histological observations indicating the accelerated development of the stressed grains is discussed.
2009-09-24 | GSE18205 | GEO
Project description:Expression data from control and drought treated wheat plants
| PRJNA344338 | ENA
Project description:Based on EST-based in silico gene expression analysis a 15k oligonucleotid microarray has been developped in order to monitor environmental stress-dependent gene expression changes in the wheat caryopsis. Using this array, the effect of water withdrawal, with and and without additional heat stress, during the first five days of grain development (0-5 DAA) has been investigated on two wheat cultivars differing in their drought sensitivity. The combined effect of heat and drought (DH) on gene expression was much significant (8-10% of the investigated genes changed >2-fold) in contrast to drought alone (1.5%). Drought and heat stress resulted in the co-ordinated change of the expression of storage proteins, some enzymes involved in sugar/starch metabolism, cell division-related and histone proteins, certain transcription factors, heat shock proteins, proteases and aquaporins. The potential link between the observed gene expression changes and the parallel histological observations indicating the accelerated development of the stressed grains is discussed. 8 samples with 2 biological replicate using dye swap
2010-05-22 | E-GEOD-18205 | biostudies-arrayexpress
Project description:In present experiment we evaluated the effects of the utrasonication of winter wheat seeds on seedling growth and development. Effect of treatment on the gene transcription and DNA methylation of seedlings were evaluated.
2022-09-14 | GSE200377 | GEO