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Pimephales promelas

ABSTRACT: Pimephales promelas FHM Transcriptome

PROVIDER: PRJNA951474 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR24044845_1.fastq.gz	Fastqsanger.gz
	SRR24044845_2.fastq.gz	Fastqsanger.gz
	SRR24044846_1.fastq.gz	Fastqsanger.gz
	SRR24044846_2.fastq.gz	Fastqsanger.gz
	SRR24044847_1.fastq.gz	Fastqsanger.gz

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Tissue-based mapping of the fathead minnow (Pimephales promelas) transcriptome and proteome

Project description:Omics approaches are broadly used to explore endocrine and toxicity-related pathways and functions. Nevertheless, there is still a significant gap in knowledge in terms of understanding the endocrine system and its numerous connections and intricate feedback loops, especially in non-model organisms. The fathead minnow (Pimephales promelas) is a widely used small fish model for aquatic toxicology and regulatory testing, particularly in North America. A draft genome has been published but the amount of available genomic or transcriptomic information is still far behind that of other more broadly studied species, such as the zebrafish. Here, we surveyed the tissue-specific proteome and transcriptome profiles in adult male fathead minnow. To do so, we generated a draft transcriptome using short and long sequencing reads. We also performed RNA sequencing and proteomics analysis on the telencephalon, hypothalamus, liver, and gut of male fish. The main purpose of this analysis was to generate tissue-specific omics data in order to support future aquatic ecotoxicogenomic and endocrine-related studies as well as to improve our understanding of the fathead minnow as an ecological model.

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