Project description:Plant growth promoting rhizobacteria (PGPR) induce positive effects in plants, such as increased growth or reduced stress susceptibility. The mechanisms behind PGPR/plant interaction are poorly understood, as most studies have described short- term responses on plants and only a few studies have analyzed plant molecular responses under PGPR colonization. Transcriptional profiles were determined by microarray analysis (Affymetrix ATH1 Genome Array) in Arabidopsis thaliana plants inoculated with the PGPR bacterial model Burkholderia phytofirmans PsJN Arabidopsis seeds were sown on square Petri dishes with half strength Murashige and Skoog medium (MS) 0.8% agar, and inoculated or not (control) with strain PsJN. To assess the effect of inactivated bacteria, an inoculum was heated at 95M-BM-0C for 20 min and then was used at the same dilution.Three biological replicates, consisting of ten plantlets of 13 days after sowing (DAS) each, for control and strain PsJN treatments, were used for global gene expression.

2013-05-31 | E-GEOD-47092 | biostudies-arrayexpress

Paraburkholderia phytofirmans OLGA172

Project description:Paraburkholderia phytofirmans OLGA172 Genome sequencing

| PRJNA226249 | ENA

Streptococcus suis T15

Project description:Streptococcus suis T15 Genome sequencing

| PRJNA206218 | ENA

Tumor T15 Sectors

Project description:The following CGH experiments were conducted on four sectors (S1-S4) from a single primary ductal carcinoma tumor (T15) using the Sector-Ploidy-Profiling (SPP) Approach. SPP involves macro-dissecting the tumor, flow-sorting nuclei by differences in total genomic DNA content and profiling the genome of the tumor subpopulations. The genomic DNA from each tumor subpopulation was labeled with Cy5 and hybridized to an 390K Rubble-Rep ROMA Microarray. A normal reference male fibroblast was labeled with Cy3 as a control. Samples were cohybridized to the same single microarray. Samples were dye-swapped and the experiments were conducted in color-reversal.

2010-05-16 | E-GEOD-16663 | biostudies-arrayexpress

Escherichia coli strain:T15

Project description:Escherichia coli strain:T15 Genome sequencing

| PRJNA309213 | ENA

Paraburkholderia phytofirmans PsJN isolate:LMG 22146

Project description:Paraburkholderia phytofirmans PsJN DAP-Seq epigenomics

| PRJNA757850 | ENA