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Effect of loss of Regnase-1 and Regnase-3 on the transcriptional profile in mouse Lin–Sca1+Kit+ (LSK) cells

ABSTRACT: Effect of loss of Regnase-1 and Regnase-3 on the transcriptional profile in mouse Lin–Sca1+Kit+ (LSK) cells

PROVIDER: PRJNA952194 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24063455_1.fastq.gz	Fastqsanger.gz
	SRR24063455_2.fastq.gz	Fastqsanger.gz
	SRR24063456_1.fastq.gz	Fastqsanger.gz
	SRR24063456_2.fastq.gz	Fastqsanger.gz
	SRR24063457_1.fastq.gz	Fastqsanger.gz

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Mx-Cre mediated deletion of SRF in murine hematopoietic LSK (Lin-Sca1+c-Kit+) cells

Project description:Analysis of hematopoietic LSK(Lin-Sca1+c-Kit+) cells lacking the Serum response factor (SRF) gene. Results provide insight into the role of SRF in regulating genetic programs important for hematopoietic stem cell development Comparison of the gene expression profile between murine Lin-Sca1+c-Kit+ cells from Mx-Cre C57BL/6 Srf WT (3) and C57BL/6 Srf KO (3). Cells were sorted by flow cytometry and RNA was harvested and hybridized to Affymetrix MOE430A.

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Effect of the Regnase-1/3-Nfkbiz axis on the epigenomic profile in mouse Flt3–CD48–Lin–Sca1+Kit+ (LSK) cells

Project description:Effect of the Regnase-1/3-Nfkbiz axis on the epigenomic profile in mouse Flt3–CD48–Lin–Sca1+Kit+ (LSK) cells

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Effect of loss of Regnase-1 and Regnase-3 on the transcriptional profile in mouse Lin–Sca1+Kit+ (LSK) cells

Project description:Regulation of lineage biases in hematopoietic stem and progenitor cells (HSPCs) is pivotal for balanced hematopoietic output. However, little is known about the mechanism behind lineage choice in HSPCs. Here, we show that mRNA decay factors Regnase-1 (Zc3h12a) and Regnase-3 (Zc3h12c) are critical for induction of myeloid lineage priming, restricting lymphoid differentiation in HSPCs. Regnase-1- and Regnase-3-mediated control of mRNA encoding Nfkbiz, a transcriptional and epigenetic regulator, was essential for balancing lymphoid/myeloid lineage output in HSPCs in vivo. Furthermore, single cell-assay for transposase-accessible chromatin sequencing (scATAC-seq) analysis revealed that Regnase-1 and Regnase-3 control the epigenetic landscape on myeloid-related gene loci in hematopoietic stem cells (HSCs) via Nfkbiz. Consistently, an antisense oligonucleotide designed to inhibit Regnase-1- and Regnase-3-mediated Nfkbiz mRNA degradation primed HSCs toward myeloid lineages by enhancing Nfkbiz expression. Collectively, the collaboration between post-transcriptional control and chromatin remodeling by the Regnase-1/Regnase-3-Nfkbiz axis governs lineage priming, instructing HSC lineage specification.

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Effect of the Regnase-1/3-Nfkbiz axis on the epigenomic profile in mouse Flt3–CD48–Lin–Sca1+Kit+ (LSK) cells

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Gene expression analysis of Cbfb-deficient LSK and GMP

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RNA-sequencing of GFP+ cells 2 days post-transduction of LSK cells with Scr or miR-99 KD lentiviral vectors

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