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VDJ analysis of IL-10+ and IL-10- splenic B1a, MZ, and FO B cells from sIgM-/- mouse

ABSTRACT: VDJ analysis of IL-10+ and IL-10- splenic B1a, MZ, and FO B cells from sIgM-/- mouse

PROVIDER: PRJNA952664 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24084700_1.fastq.gz	Fastqsanger.gz
	SRR24084700_2.fastq.gz	Fastqsanger.gz
	SRR24084701_1.fastq.gz	Fastqsanger.gz
	SRR24084701_2.fastq.gz	Fastqsanger.gz
	SRR24084702_1.fastq.gz	Fastqsanger.gz

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VDJ analysis of IL-10+ and IL-10- splenic B1a, MZ, and FO B cells from sIgM-/- mouse

Project description:We compared the clonotypes of IL-10+ and IL-10- B1a, marginal zone and follicular B cells from the spleen of sIgM-/-IL10GFP mouse using 10X single cell RNAseq to analyze potential differences in clonality between IL-10+ and IL-10- B cells.

2023-11-15 | GSE229063 | GEO

miRNA expression profile of follicular (FO) and marginal zone (MZ) B cells

Project description:We have analyzed miRNA expression profile in FO and MZ B cells to identified differentially expressed miRNAs between this two subsets that could be potencially involved in the regulation of terminal B cell differentiation Spleens from CD19-Creki/+Dicerfl/+ and CD19-Creki/+Dicerfl/fl mice were collected and after erythrocyte lysis, splenocytes were stained with anti-B220, anti-CD21 and anti-CD23 antibodies. B220+CD21brightCD23+ (MZ) and B220+CD21+CD23bright (FO) B cell populations were sorted and total RNA of purified populations was extracted with TRIzol. miRNA microarray hybridations were performed on Mouse miRNA Microarray platform (Agilent Technologies).

2011-11-01 | E-GEOD-25139 | biostudies-arrayexpress

miRNA expression profile of follicular (FO) and marginal zone (MZ) B cells

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RNA-seq analysis of follicular (FO) and marginal zone (MZ) B cells, isolated from TLR9WT or TLR9-/- BALB/c mice, stimulated with TLR7 agonists (CL097, Invivogen) or left unstimulated .

Project description:Purpose: The goal of this study was to assess if increased TLR7 signaling could be seen in TLR9-/- compared to TLR9WT FO and MZ B cells. Methods : FO and MZ B cells from TLR9WT or TLR9-/- BALB/c mice were sorted using FACSaria. A minimum of 100,000 cells per B cell subsets were sorted. Sorted B cell subsets were stimulated for 4 hours at 0.25M/ml with TLR7 agonist CL097 (Invivogen) at 5µg/ml or left unstimulated. RNA was isolated using the RNeasy Plus Micro Kit (QIAGEN). Samples were sequenced on an Illumina Next-seq 2000 using a P3 200 cycle flowcell (Illumina, Inc) with 2 x 101 bp paired-end reads (20 million reads per sample). Results: There were no significant DEG (FDR > 0.05) following TLR7 stimulation between TLR9WT and TLR9-/- FO or MZ B cells.

2022-08-03 | GSE202105 | GEO

IL-10

Project description:IL-10

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Transcriptome of Zfp36l1-deficient MZ B cells, WT MZ B cells and WT FO B cells.

Project description:Purpose: to identify the effects on the transcriptome of deleting ZFP36L1 in MZ B cells

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Project description:Transcriptional profiling and gene expression profiling analysis of sorted CD8+IL-10+ T cells compared to CD8+IL-10- T cells using IL-10-GFP(tiger) reporter mice

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Expression data from IL-10+ and IL-10- CD8 T cells

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CD8+ T cells: IL-10+ VS IL-10-

Project description:Transcriptional profiling and gene expression profiling analysis of sorted CD8+IL-10+ T cells compared to CD8+IL-10- T cells using IL-10-GFP(tiger) reporter mice Two sample, CD8+IL-10+ T cells vs CD8+IL-10- T cells. Three replicate per array.

2013-06-21 | E-GEOD-37739 | biostudies-arrayexpress

IL-10+ T cells compared to IFNg+/IL-10- T cells induced by IL-10 tolerogenic dendritic cells

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