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Shigella flexneri

ABSTRACT: Shigella flexneri Transcriptome or Gene expression

PROVIDER: PRJNA952739 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24100153_1.fastq.gz	Fastqsanger.gz
	SRR24100153_2.fastq.gz	Fastqsanger.gz
	SRR24100154_1.fastq.gz	Fastqsanger.gz
	SRR24100154_2.fastq.gz	Fastqsanger.gz
	SRR24100155_1.fastq.gz	Fastqsanger.gz

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Transcriptomic profiling of HeLa cells infected with Shigella flexneri

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Transcriptional Profile of the Shigella flexneri Response to Berberine

Project description:Berberine is a natural isoquinoline alkaloid found in Chinese medicinal herbs which is active against a variety of microbial infections. To examine the potential effects of berberine on Shigella flexneri, a whole-genome DNA microarray was constructed and transcriptome analysis of the cellular responses of S.flexneri when exposed to Berberine Chloride (BC) was performed.

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Dual-transcriptome of Shigella flexneri infecting human HCT116 cells

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The expression profiles analysis of a virulence plasmid-cured strain of shigella flexneri

Project description:In this study, we analyzed the expression profiles of a virulence plasmid-cured strain and wild-type strain of shigella flexneri. The results showed that the genes of glp regulon were upregulated in mutant bacteria in stationary phase cultures.

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Expression analysis of Shigella flexneri 2a strain 301

Project description:Investigation of whole genome gene expression to identify overlooked sRNAs and sORFs. Background The completion of numerous genome sequences has introduced an era of whole-genome study. However, many real genes, including small RNAs (sRNAs) and small ORFs (sORFs), are missed in genome annotation. In order to improve genome annotation, we sought to identify novel sRNAs and sORFs in Shigella, the principal etiologic agents of bacillary dysentery or shigellosis. Results Firstly, we identified 64 sRNAs in Shigella which is experimentally validated in other bacteria based on sequence conservation. Secondly, among possible approaches to search for sRNAs, we employed computer-based and tiling array based methods, followed by RT-PCR and northern blots. This allowed us to identify 12 sRNAs in Shigella flexneri strain 301. We also find 29 candidate sORFs. Conclusions This investigation provides an updated and comprehensive annotation of the Shigella genome, increases the expected numbers of sORFs and sRNAs with the corresponding impact on future functional genomics and proteomics studies. Our method can be used for the large scale reannotation of sRNAs and sORFs in any microbe whose genome sequence is available.

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Shigella flexneri strain:2457T

Project description:Shigella flexneri strain:2457T Transcriptome or Gene expression

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Shigella flexneri strain:301

Project description:Shigella flexneri strain:301 Transcriptome or Gene expression

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