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ABSTRACT: Rumen epithelial mRNA of Tibetan sheep

PROVIDER: PRJNA954266 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24143925_1.fastq.gz	Fastqsanger.gz
	SRR24143925_2.fastq.gz	Fastqsanger.gz
	SRR24143926_1.fastq.gz	Fastqsanger.gz
	SRR24143926_2.fastq.gz	Fastqsanger.gz
	SRR24143927_1.fastq.gz	Fastqsanger.gz

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Project description:Tibetan sheep rumen epithelial tissue microRNA sequencing

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The mechanism of promoting proliferation of rumen epithelial cells in Tan sheep by adding succinate to the diet based on the interaction between rumen microbiota and host.

Project description:A healthy rumen is crucial for normal growth and improved production performance of ruminant animals. Rumen microbes participate in and regulate rumen epithelial function, and the diverse metabolites produced by rumen microbes are important participants in rumen microbe-host interactions. SCFAs, as metabolites of rumen microbes, have been widely studied, and propionate and butyrate have been proven to promote rumen epithelial cell proliferation. Succinate, as an intermediate metabolite in the citric acid cycle, is a final product in the metabolism of certain rumen microbes, and is also an intermediate product in the microbial synthesis pathway of propionate. However, its effect on rumen microbes and rumen epithelial function has not been studied. It is unclear whether succinate can stimulate rumen epithelial development. Therefore, in this experiment, Chinese Tan sheep were used as experimental animals to conduct a comprehensive analysis of the rumen microbiota community structure and rumen epithelial transcriptome, to explore the role of adding succinate to the diet in the interaction between the rumen microbiota and host.

2023-06-12 | GSE233696 | GEO

Project description:Rumen microorganisms of Tibetan sheep

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Project description:Rumen microorganisms of Tibetan sheep

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Community composition of plant biomass-associated bacteria in sheep rumen

Project description:Microbiome DNA from the adhering fraction of a sheep rumen. The RSTs were generated using an improved version of SARST (referred to as iSARST) from the microbiome DNA extracted from the adhering fraction of the rumen content taken from a sheep. The iSARST method is going to be submitted to Nature Biotechnology for publication. Keywords: other

2004-10-15 | E-GEOD-1842 | biostudies-arrayexpress

Community composition of plant biomass-associated bacteria in sheep rumen

2004-10-15 | GSE1842 | GEO

RNA seq of ventral rumen wall of Australian sheep

Project description:Experiment to understand relationships between sheep rumen wall transcriptome and microbial methane emissions

2017-12-30 | GSE81847 | GEO

Project description:Rumen bacterial diversity and function on Tibetan sheep

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Experimental validation of HiSpOD probe design with complex background mRNA or 3 known antiense RNA and expression analysis on a contaminated groundwater sample

Project description:HiSpOD is a new efficient functional microarrays probe design algorithm especially dedicated for the microbial ecology and environmental studies. It was used to design 3392 probes targeting 21 genes involved in chlorinated solvent biodegradation pathways and synthesized on a nimblegen microarray. In order to test the probe specificity, the microarray was firstly hybridized to 6 M-BM-5g of labelled aRNA from sheep rumen content (background aRNA). Secondly, hybridization of 1011 copies of labelled aRNA derived from in vitro transcription of three synthetic genes (mmoC, vcrA and tceA) and mixed with 6 M-BM-5g of the same complex background material were performed to test their sensibility. Finally, the expression analysis of a contaminated groundwater sample was performed. A 3 chip study was realized. The first one is a negative control performed with a complex background material (labelled antisense mRNA from sheep rumen content). The second one is a positive control realized with labelled antisense RNA derived from in vitro transcription of three synthetic genes mixed the same complex background material. The third consists in the hybridization of antisense mRNA retrieved from a contaminated groundwater. Each probe (3392) was synthetized in triplicate, and a total of 8,863 random probes was used to determine the background noise.

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Project description:Rumen epithelial tissue methylome of sheep

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