Project description:The goal of this study was to compare the skin transcriptomes of non-irradiated and 30 Gy irradiated Pelophylax nigromaculatus and to find the polypeptide sequences that were changed before and after irradiation. Skin mRNA profiles of non-irradiated and 30Gy irradiated Pelophylax nigromaculatus were generated by deep sequencing,using Illumina HiSeq.The sequenced sequences were assembled into transcripts, and the transcripts were hierarchically clustered using the Corset program. Subsequent analysis was performed using the clustered sequences as references:CDS prediction, SNP and InDel analysis, gene expression level analysis, SSR analysis, RNA-seq overall quality assessment, differential expression analysis, differential gene GO enrichment analysis and KEGG enrichment analysis.Through differential analysis, 875 down-regulated mRNAs and 536 up-regulated mRNAs were identified, and 271 significant changes in peptides were found through the rank sum test, which provided the basis for the next study.
Project description:To compare the similarities and differences in species diversity of the gut microbiota between the patients with melasma and healthy subjects. The feces were collected for 16S rRNA sequencing analysis of the gut microbiota.
Project description:Gut microbiota were assessed in 540 colonoscopy-screened adults by 16S rRNA gene sequencing of stool samples. Investigators compared gut microbiota diversity, overall composition, and normalized taxon abundance among these groups.
Project description:Insect gut microbiota plays important roles in acquiring nutrition, preventing pathogens infection, immune responses, and communicating with the environment. Gut microbiota can be affected by some external factors such as foods, temperature, and antibiotics. Spodoptera frugiperda (Lepidoptera: Noctuidae) is an important destructive pest of grain crops all over the world. The function of gut microbiota in S. frugiperda remains to be investigated. In this study, we fed the S. frugiperda with the antibiotic mixture (penicillin, gentamicin, rifampicin, and streptomycin) to perturb the gut microbiota, and further examined the effect of dysbiosis in gut microbiota on the gene expression of S. frugiperda by RNA sequencing. We found the composition and diversity of the gut bacterial community were changed in S. frugiperda after antibiotics treatmen, and the expression of genes related to energy and metabolic process were affected after antibiotics exposure in S. frugiperda. Our work will help understand the role of gut microbiota in insects.
Project description:Interventions: No endometrial lesions group:Nil;Endometrial carcinoma group:Nil
Primary outcome(s): Diversity of gut microbiota
Study Design: Factorial
