Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Symmetry breaking in gastruloid development

ABSTRACT: Symmetry breaking in gastruloid development

PROVIDER: PRJNA954812 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Symmetry breaking in gastruloid development

Project description:Symmetry breaking in gastruloid development

| PRJNA759809 | ENA

Symmetry breaking in gastruloid development

Project description:We use single cell RNA sequencing to describe the transcriptional changes during gastruloid development from 0 to 120h hours.

2023-04-29 | GSE229513 | GEO

Symmetry breaking in gastruloid development

Project description:We use single cell RNA sequencing to describe the transcriptional changes during gastruloid development from 24 to 84 hours with 12 hours intervals.

2021-09-04 | GSE183255 | GEO

Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking

Project description:Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking

| PRJNA1146327 | ENA

Multimodal characterization of murine gastruloid development

Project description:Gastruloids are highly scalable, three-dimensional assemblies generated from pluripotent stem cells that recapitulate fundamental principles of embryonic pattern formation in vitro. Using single cell RNA and multiome sequencing we provide a comprehensive resource mapping cellular states and cell types found during gastruloid development and compare them to the in vivo embryo. We further develop a high throughput gastruloid handling and imaging pipeline to spatially monitor cell type emergence and unfolding of symmetry breaking during gastruloid development. We report spatial variability of pluripotency states in early gastruloids that determines a binary cell response to Wnt activation. While cells situated in the core of the gastruloid revert to an ectopic pluripotent state, peripheral cells differentiate to a primitive streak like state. These two populations then cause gastruloids to break radial symmetry, allowing axial elongation and commitment to the three embryonic germ layers. Finally by performing a phenotypic compound screen, we perturb thousands of gastruloids at relevant developmental time points deriving a phenotypic landscape and inferring molecular regulator networks underlying gastruloid development. Employing this resource, we improve the formation of anterior structures in the existing gastruloid model, using a dual Wnt modulation approach to differentiate an anterior ectopic pluripotent core to anterior ecto- and endodermal structures. This work gives is a resource to understand how gastruloids develop and, more generally, how homogenous cell populations can generate complex patterns in vitro.

2023-09-07 | GSE229386 | GEO

Self-organization and symmetry breaking in intestinal organoid development

Project description:Self-organization and symmetry breaking in intestinal organoid development

| PRJNA476588 | ENA

Self-organization and symmetry breaking in intestinal organoid development [scRNA-Seq]

Project description:Intestinal organoids are complex three-dimensional structures that mimic cell type composition and tissue organization of the intestine by recapitulating the self-organizing capacity of cell populations derived from a single stem cell. Crucial in this process is a first symmetry-breaking event, in which only a fraction of identical cells in a symmetrical cyst differentiate into Paneth cells, which in turn generates the stem cell niche and leads to asymmetric structures such as crypts and villi. We here combine a quantitative single-cell gene expression and imaging approach to characterize the development of intestinal organoids from a single cell. We show that intestinal organoid development follows a regeneration process driven by transient Yap1 activation. Cell-to-cell variability in Yap1, emerging in symmetrical cysts, initiates a Notch/Dll1 lateral inhibition event driving the symmetry-breaking event and the formation of the first Paneth cell. Our findings reveal how single cells exposed to a uniform growth-promoting environment have the intrinsic ability to generate emergent, self-organized behavior resulting in the formation of complex multicellular asymmetric structures.

2019-04-25 | GSE115956 | GEO

Self-organization and symmetry breaking in intestinal organoid development [bulk RNA-Seq]

2019-04-25 | GSE115955 | GEO

Self-organization and symmetry breaking in intestinal organoid development [scRNA-Seq]

Project description:Self-organization and symmetry breaking in intestinal organoid development [scRNA-Seq]

| PRJNA476590 | ENA

Self-organization and symmetry breaking in intestinal organoid development [bulk RNA-Seq]

Project description:Self-organization and symmetry breaking in intestinal organoid development [bulk RNA-Seq]

| PRJNA476589 | ENA