Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transglutaminase 2 regulates terminal erythroid differentiation via cross-linking activity

ABSTRACT: Transglutaminase 2 regulates terminal erythroid differentiation via cross-linking activity

PROVIDER: PRJNA955100 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transglutaminase 2 regulates terminal erythroid differentiation via cross-linking activity

Project description:To gain the whole picture of terminal erythroid differentiation, bone marrow erythroblasts of different maturation stages including proerythroblasts (Pro),basophilic erythroblasts (Baso), polychromatic erythroblasts (Poly) and orthochromatic erythroblasts (Ortho) were isolated and sorted.Transcriptomic analysis of these four stages of cells was performed.

2023-04-23 | GSE229589 | GEO

two steps cross-linking ChIP-seq

Project description:two steps cross-linking ChIP-seq

| PRJNA794157 | ENA

single step cross-linking ChIP-seq

Project description:single step cross-linking ChIP-seq

| PRJNA794167 | ENA

Downregulation of Myc is essential for terminal erythroid maturation

Project description:Terminal differentiation of mammalian erythroid progenitors involves 4-5 cell divisions and induction of many erythroid important genes, followed by chromatin and nuclear condensation and enucleation. The protein levels of c-myc (Myc) are reduced dramatically during late stage erythroid maturation, coinciding with cell cycle arrest in G1-phase and enucleation, suggesting possible roles for c-myc in either or both of these processes. Here we demonstrate that ectopic Myc expression affects terminal erythroid maturation in a dose-dependent manner. Expression of Myc at physiological levels did not affect erythroid differentiation or cell cycle shutdown, but specifically blocked erythroid nuclear condensation and enucleation. Myc prevented deacetylation of several lysine residues in histones H3 and H4 that are normally deacetylated during erythroid maturation. When over-expressed at levels higher than the physiological range, Myc blocked erythroid differentiation and the cells continued to proliferate in cytokine-free, serum-containing culture medium with an early erythroblast morphology. These studies reveal an important dose-dependent function of Myc in regulating terminal maturation in mammalian erythroid cells. Our findings further support the emerging notion that Myc regulates chromatin structure by regulating global histone acetylation states. Five groups with three biological replicates in each.

2010-10-13 | E-GEOD-18558 | biostudies-arrayexpress

Two steps cross-linking LCL ChIP-seq

Project description:Two steps cross-linking LCL ChIP-seq

| PRJNA795780 | ENA

Single step cross-linking LCL ChIP-seq

Project description:Single step cross-linking LCL ChIP-seq

| PRJNA795781 | ENA

Downregulation of Myc is essential for terminal erythroid maturation

2010-10-13 | GSE18558 | GEO

Tissue Transglutaminase 2 has higher affinity for relaxed than for stretched fibronectin fibers

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the interaction between fibronectin (Fn; full-length or gelatin-binding domain fragment = Fn 45) with tissue transglutaminase 2 in oxidized and non-oxidized form. The complexes were cross-linked with disuccinimidyl suberate (DSS) or a combination of pimelic dihydrazide (PDH) in combination with the zero-length cross-linking reagent, DMTMM.

2023-12-20 | PXD043976 | Pride

Global Transcriptome Analyses of Mammalian Terminal Erythroid Differentiation

Project description:Global Transcriptome Analyses of Mammalian Terminal Erythroid Differentiation

| PRJNA234281 | ENA

Codanin-1 Mutations Engineered in Human Erythroid Cells Demonstrate Role of CDAN1 in Terminal Erythroid Maturation

Project description:CDAN1, and residue R1042, have a function in DNA replication and organization in terminal erythroid maturation.

2021-03-31 | GSE151476 | GEO