Project description:Gene expression profiling of whole epididymis in embryonic (gestational day 12, 14, 16 & 18) and postnatal day 2 mice. (GUDMAP Series ID: 4)
Project description:Gene expression profiling of whole ovary in embryonic (gestional day 11, 12, 14, 16 & 18) and postnatal day 2 mice. (GUDMAP Series ID: 5)
Project description:To investigate the role of HSF5 protein in mouse reproduction, We collected WT or Hsf5+/- (control) and Hsf5-/- testes at postnatal day 16 (P16) for RNA sequencing.
Project description:Findings from gene expression studies can help identify relevant biological pathways. RNA from three pairs of testes from wild-type and pcd3J-/- mice at postnatal day 18 was subjected to a genome-scale gene expression analysis using the Affymetrix GeneChip Mouse Gene 1.0 ST Array, which includes approximately 28,000 genes. RNA from postnatal day 18 was used to minimize the changes in gene expression that might occur in response to pathological changes in the pcd3J-/- mice, because postnatal day 18 was the earliest time point at which structural differences between testes from wild-type and pcd3J-/- mice were observed via histopathology.Three pcd3J-/- mutant mice and three corresponding wild-type (pcd3J+/+) littermates at postnatal day 18 were used for the microarray analyses.
Project description:Pregnant rats were received i.p. injection of L‑NAME (250 mg/kg/day) from gestational day 15 to 20 to establish preeclampsia model. Proteome analysis of cerebrospinal fluid on postnatal day 5 were performed on male offspring.
Project description:ZFP541 is one of the candidates for a transcriptional regulator during mouse meiotic prophase. In order to address the role of ZFP541, transcriptomes of meiotic prophase-enriched population were compared between Zfp541 +/- and Zfp541 KO testes by RNA-seq. The meiotic prophase-enriched population was isolated from Zfp541 +/- and Zfp541 KO testes at postnatal day 18 (P18), on the Rec8-3FH-GFP KI background by fluorescent sorting of GFP positive cells.
