Project description:To screen the genes and pathways regulated by BRD3 in the THP1 monocytes induced by LPS, THP1 monocytes were transfected by BRD3shRNA and then treated by LPS for 3 hours.
Project description:To screen the genes regulated by BRD3 in the THP1 monocytes induced by LPS, THP1 monocytes were treated by LPS for 1 hour in the presence or absence of OTX015
Project description:To screen the genes and pathways regulated by CREB1 in the THP1 monocytes induced by LPS, THP1 monocytes were transfected by CREB1 shRNA and then treated by LPS for 3 hours.
Project description:In order to further explore the expression of lncrna in asthma, we used lipopolysaccharide (LPS) to activate human monocyte macrophage THP-1, and then used deep sequencing method to detect the expression of lncrna in activated and inactive THP1 cells. The results showed that there were different expression of lncrna in LPS activated and inactive THP1 cells
Project description:CD14+ Monocytes from healthy volunteers were purified by MACS (negative selection) and FACSorting and either left untreated or stimulated for 24h and 48h with LPS. THP-1 cells were stimulated for 4h, 24h and 48h with LPS. Glycoproteins were captured with hydrazide chemistry and tryptic and PNGase F-released peptide fractions analyzed by MS/MS. Quantitative assessment revealed differential glycoprotein expression in activated/LPS-tolerized monocytes and naïve monocytes and THP-1 cells.