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Enterobacter

ABSTRACT: Studies on colistin resistance Enterobacter spp. from Kolkata, India

PROVIDER: PRJNA957587 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR25180905_1.fastq.gz	Fastqsanger.gz
	SRR25180905_2.fastq.gz	Fastqsanger.gz
	SRR28212230_1.fastq.gz	Fastqsanger.gz
	SRR28212230_2.fastq.gz	Fastqsanger.gz

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Enterobacter

Project description:Studies on carbapenem resistance Enterobacter spp.during 2023 from Kolkata ,India

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Enterobacter roggenkampii

Project description:Novel colistin resistance gene from Enterobacter roggenkampii

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Enterobacter cloacae strain:ST-335 | isolate:S45

Project description:Colistin-resistance in Enterobacter cloacae

| PRJNA401380 | ENA

Aminoglycoside heteroresistance in Enterobacter cloacae is driven by the cell envelope stress response.

Project description:Enterobacter cloacae is a Gram-negative nosocomial pathogen of the ESKAPE (Enterococcus, Staphylococcus, Klebsiella, Acinetobacter, Pseudomonas, and Enterobacter spp.) priority group with increasing multi-drug resistance via the acquisition of resistance plasmids. However, E. cloacae can also display forms of antibiotic refractoriness, such as heteroresistance and tolerance. Here, we report that E. cloacae displays transient heteroresistance to aminoglycosides, which is accompanied with the formation of small colony variants (SCVs) with increased minimum inhibitor concentration (MIC) of gentamicin and other aminoglycosides used in the clinic, but not other antibiotic classes. To explore the underlying mechanisms, we performed RNA sequencing of heteroresistant bacteria, which revealed global gene-expression changes and a signature of the CpxRA cell envelope stress response. Deletion of the cpxRA two-component system abrogated aminoglycoside heteroresistance and SCV formation, pointing to its indispensable role in these processes. The introduction of a constitutively active allele of cpxA led to high aminoglycoside MICs, consistent with cell envelope stress response driving these behaviours in E. cloacae. Cell envelope stress can be caused by environmental cues, including heavy metals. Indeed, bacterial exposure to copper increased gentamicin MIC in the wild-type, but not in the ΔcpxRA mutant. Moreover, copper exposure also elevated the gentamicin MICs of clinical isolates from bloodstream infections, suggesting that CpxRA- and copper-dependent aminoglycoside resistance is broadly conserved in E. cloacae strains. Altogether, we establish that E. cloacae relies on transcriptional reprogramming via the envelope stress response pathway for transient resistance to a major class of frontline antibiotic.

2023-10-18 | GSE236124 | GEO

Enterobacter

Project description:colistin-resistant Enterobacter cloacae complex

| PRJDB13693 | ENA

Enterobacter asburiae

Project description:Whole genome sequence of antibiotic resistance Enterobacter spp.

| PRJDB10973 | ENA

Colistin heteroresistance in Enterobacter cloacae is regualted by PhoPQ-dependent 4-amino-4-deoxy-L arabinose addition to lipid A

Project description:We report the transcriptional expression from wild type, ΔphoPQ, and ΔpmrAB to understand their contribution to colistin resistance.

2019-06-20 | GSE127802 | GEO

Drug efflux and lipid A modification by 4-L-aminoarabinose are key mechanisms of polymyxin B resistance in the sepsis pathogen Enterobacter bugandensis

Project description:To investigate the mechanism of PmB resistance and heteroresistance in Enterobacter bugandensis by analysing the differential gene expression under PmB treatment.

2024-05-01 | GSE218614 | GEO

Enterobacter

Project description:Study on blood-borne Carbapenem-resistant Enterobacter cloacae complex isolated from sepsis patients admitted to ICU departments of tertiary care hospitals at Kolkata,India.

| PRJNA721536 | ENA

Cefazolin and Imipenem Enhance AmpC Expression and Resistance in NagZ-dependent manner in Enterobacter cloacae complex

Project description:Background: Enterobacter cloacae complex (ECC) is a common opportunistic pathogen and is responsible for causing various infections in humans. Owing to its inducible chromosomal AmpC β-lactamase (AmpC), ECC is inherently resistant to the 1st- and 2nd- generation cephalosporins. However, whether β-lactams antibiotics enhance ECC resistance remains unclear. Results: In this study, we found that subinhibitory concentrations (SICs) of cefazolin (CFZ) and imipenem (IMP) can advance the expression of AmpC and enhance its resistance towards β-lactams through NagZ in Enterobacter cloacae (EC). Further, AmpC manifested a substantial upregulation in EC in response to SICs of CFZ and IMP. In nagZ knockout EC (ΔnagZ), the resistance to β-lactam antibiotics was rather weakened and the effect of CFZ and IMP on AmpC induction was completely abrogated. NagZ ectopic expression can rescue the induction effects of CFZ and IMP on AmpC and increase ΔnagZ resistance. More importantly, CFZ and IMP have the potential to induce the expression of AmpR's target genes in a NagZ-dependent manner. Conclusions: Our findings suggest that NagZ is a critical determinant for CFZ and IMP to promote AmpC expression and resistance and that CFZ and IMP should be used with caution since they may aggravate ECC resistance. At the same time, this study further improves our understanding of resistance mechanisms in ECC.

2022-07-01 | GSE207144 | GEO

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