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ABSTRACT: Bovine tuberculosis caused by Mycobacterium orygis

PROVIDER: PRJNA958208 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24255213_1.fastq.gz	Fastqsanger.gz
	SRR24255213_2.fastq.gz	Fastqsanger.gz
	SRR24255214_1.fastq.gz	Fastqsanger.gz
	SRR24255214_2.fastq.gz	Fastqsanger.gz

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Project description:Tuberculosis caused by Mycobacterium orygis in wild ungulates in Chennai, South India

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Project description:①Background:Tuberculosis is mainly a respiratory tract infection caused by mycobacterium tuberculosis and one of the leading causes of death worldwide. According to the Global Tuberculosis Report in 2021, About a quarter of the world's population is infected with Mycobacterium tuberculosis and China is the second highest burden of TB. Although TB diagnosis and prevention techniques have become more mature, the number of TB cases is still increasing, mainly due to: the prevalence of drug-resistant tuberculosis bacteria, tuberculosis and HIV co-infection, long incubation time of mycobacterium tuberculosis difficult to early diagnosis and so on. Therefore, it is of great significance to study the pathogenesis of mycobacterium tuberculosis infection.②Method: THP-1 cells were treated with 50ng/ml PMA for 24 hours, so that THP-1 cell can be induced into macrophages. After that THP-1 macrophages were infected with mycobacterium tuberculosis H37Rv(MOI=1), which were collected and applied to RNA-sequencing. The constructed sequencing library was sequenced using an Illumina Novaseq 6000 system.

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