Project description:In this study, the complete mitochondrial genomes of Myzornis pyrrhoura and Fulvetta vinipectus were sequenced and described for the first time. The whole mitochondrial genomes of M. pyrrhoura and F. vinipectus are 17,397 bp and 16,961 bp in length, with the G + C percentage 46.34% and 47.36%, respectively. Both genomes contain 13 protein-coding genes, 22 transfer RNA genes, 2 ribosome RNA genes, and 1 non-coding control region. The arrangement of genes is identical to mitochondrial genomes of Sylviidae species reported previously. A phylogenetic reconstruction supported that M. pyrrhoura and F. vinipectus are members of family Sylviidae. The mitochondrial genomes of these two species reported here would be helpful in better understanding the phylogeny and evolution of Sylviidae.
Project description:modENCODE_submission_5986 This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. Our 126 strategically selected targets include RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents. We will integrate information generated with existing knowledge on the biology of the targets and perform ChIP-seq analysis on mutant and RNAi extracts lacking selected target proteins. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: L3 Larva; Genotype: wild type; Sex: mixed Male and Hermaphrodite population; EXPERIMENTAL FACTORS: Developmental Stage L3 Larva; temp (temperature) 20 degree celsius; Strain N2; Antibody NURF-1 SDQ3525 (target is NURF-1)