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ABSTRACT: KPC mice on ketogenic diet versus standard chow diet

PROVIDER: PRJNA961745 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24300504_1.fastq.gz	Fastqsanger.gz
	SRR24300504_2.fastq.gz	Fastqsanger.gz
	SRR24300505_1.fastq.gz	Fastqsanger.gz
	SRR24300505_2.fastq.gz	Fastqsanger.gz
	SRR24300506_1.fastq.gz	Fastqsanger.gz

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Project description:Analysis of liver gene transcription during feeding of a ketogenic diet. Ketogenic diets may alter physiologic and metabolic profiles in a direction that favors weight loss. C57BL/6J mice were maintained for six weeks on either chow or ketogenic diet. Mice eating KD had lower weights, 90% reduction in insulin levels and increased energy expenditure compared to animals fed chow. Despite consumption of a very high fat diet serum lipids remained normal. Here we show that consumption of KD shifted liver metabolism to drastically increased fatty acid oxidation. Concurrently, expression of genes involved in fatty acid synthesis were markedly suppressed. Reference: A high fat, ketogenic diet induces a unique metabolic state in mice. Kennedy AR, Pissios P, Out H, Xue B, Asakura K, Furukawa N, Marino FE, Liu FF, Kahn BB, Liberman TA, Maratos-Flier E. in press, 2007, Am J Physiol Metab 292. Experiment Overall Design: Eight week old C57BL/6 mice were fed either chow (Labdiet 5008, Pharmserv) or KD (F3666, Bio-Serv) for six weeks. Livers were harvested in the morning in ad lib fed animals. Total RNA from 2-3 animals in each group was used for Affymetrix analysis.

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