Establishment and characterization of canine mammary gland tumor(MGT) cell lines
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA962052 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24600320_1.fastq.gz | Fastqsanger.gz | |||
| SRR24600320_2.fastq.gz | Fastqsanger.gz | |||
| SRR24600321_1.fastq.gz | Fastqsanger.gz | |||
| SRR24600321_2.fastq.gz | Fastqsanger.gz | |||
| SRR24600322_1.fastq.gz | Fastqsanger.gz |
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Project description:To investigate the canine mammary gland tumor, we established 8 cell lines We then performed gene expression profiling analysis using data obtained from RNA-seq of 10 different cells.
2024-04-30 | GSE230271 | GEO
Project description:Purpose: the aim of this study was to characterize cell lines derived from canine mammary gland neoplasia which could be further used for basic and applied oncology research. Methods: samples of canine mammary carcinomas were taken to primary culture and two cell lines were well-established and described in terms of tumorigenesis, cell morphology and gene expression profile through RNA-seq analysis. Results: Both cell lines presented spindle-shape morphology and shown common malignancies features such as invasion potential and co-expression of cytoskeleton proteins. Also, we found dysregulations in gene expression patterns between the two cell cultures as well as compared to normal mammary gland tissue. Conclusion: transcriptomic profile of these cell lines contributed to the characterization of potential prognostic and predictive biomarkers and may enable the development of possible therapeutic molecular targets.
2017-07-01 | GSE98482 | GEO
Project description:Canine mammary gland tumors (CMGTs) are the most common neoplasms in sexually intact female dogs. CMGTs have been suggested as a model for studying human breast cancer because of several similarities, including the relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdcscid/J mouse at the site of subcutaneous SNP cell injection, with an average size at 7 days after inoculation of 49.9 mm3. SNP cells are characterized by proliferation in a tubulopapillary pattern. Moreover, we examined miRNA expression in the cultured cells and found that the expression values of miRNA-143 and miRNA-138a showed the greatest increase and decrease, respectively, of all miRNAs observed. These miRNAs might therefore play a significant role in the malignancy of SNP cells. SNP cells might serve as a model for future genetic analysis and clinical treatments of human breast tumors.
2016-01-01 | GSE67129 | GEO
Project description:Molecular mechanisms of the canine mammary malignancy. RNA isolated from canine mammary tumours of the 3rd grade of malignancy was pooled and hybrydized on the same microarray as RNA isolated from canine mammary tumours of the 1st grade of malignancy. Based on the results the "malignancy portrait" was assessed.
2013-10-01 | GSE44033 | GEO
Project description:[original title] Metastatic Canine Mammary Carcinomas can be Identified by a Gene Expression Profile that partly Overlaps with Human Breast Cancer Profiles. Introduction: Similar to human breast cancer mammary tumors of the female dog are commonly associated with a fatal outcome due to the development of distant metastases. However, the molecular defects leading to metastasis are largely unknown and the value of canine mammary carcinoma as a model for human breast cancer is unclear. In this study, we analyzed the gene expression signatures associated with mammary tumor metastasis and asked for parallels with the human equivalent. Methods: Messenger RNA expression profiles of twenty-seven lymph node metastasis positive or negative canine mammary carcinomas were established by microarray analysis. Differentially expressed genes were functionally characterized and associated with molecular pathways. The findings were also correlated with published data on human breast cancer. Results: Metastatic canine mammary carcinomas had 1,011 significantly differentially expressed genes when compared to non-metastatic carcinomas. Metastatic carcinomas had a significant up-regulation of genes associated with cell cycle regulation, matrix modulation, protein folding and proteasomal degradation whereas cell differentiation genes, growth factor pathway genes and regulators of actin organization were significantly down-regulated. Interestingly, 265 of the 1,011 differentially expressed canine genes are also related to human breast cancer and, vice versa, parts of a human prognostic gene signature were identified in the expression profiles of the metastatic canine tumors. Conclusions: Metastatic canine mammary carcinomas can be discriminated from non-metastatic carcinomas by their gene expression profiles. More than one third of the differentially expressed genes are also described of relevance for human breast cancer. Many of the differentially expressed genes are linked to functions and pathways which appear to be relevant for the induction and maintenance of metastatic progression and may represent new therapeutic targets. Furthermore, dogs are in some aspects suitable as a translational model for human breast tumors in order to identify prognostic molecular signatures and potential therapeutic targets.
2010-12-01 | GSE20718 | GEO
Project description:Spontaneously occurring canine mammary cancer represents an excellent model of human breast cancer, but is greatly understudied. To better use this valuable resource, we performed whole-genome sequencing, whole-exome sequencing, RNA-seq, and/or high-density arrays on twelve canine mammary cancer cases, including seven simple carcinomas and four complex carcinomas. Canine simple carcinomas, which histologically match human breast carcinomas, harbor extensive genomic aberrations, many of which faithfully recapitulate key features of human breast cancer. Canine complex carcinomas, which are characterized by proliferation of both luminal and myoepithelial cells and are rare in human breast cancer, seem to lack genomic abnormalities. Instead, these tumors have about 35 chromatin-modification genes downregulated and are abnormally enriched with active histone modification H4-acetylation, whereas aberrantly depleted with repressive histone modification H3K9me3. Our findings indicate the likelihood that canine simple carcinomas arise from genomic aberrations, whereas complex carcinomas originate from epigenomic alterations, reinforcing their unique value. Canine complex carcinomas offer an ideal system to study myoepithelial cells, the second major cell lineage of the mammary gland. Canine simple carcinomas, which faithfully represent human breast carcinomas at the molecular level, provide indispensable models for basic and translational breast cancer research.
2014-09-07 | GSE54535 | GEO
Project description:Spontaneous mammary tumours are the most prevalent type of neoplasm in women as well as in female dogs. Even though ovarian hormones, mainly estrogens and progestins, are known to play a key role in mammary tumorigenesis, conflicting reports have been obtained from in vivo and in vitro studies concerning their role, especially of progestins, in mammary tumorigenesis. Prolonged exposure to high levels of progestins during unusually long luteal phase of oestrous cycle is suspected to be the key event in canine mammary tumorigenesis. Accordingly, our previous studies have shown development of mammary hyperplasia in dogs upon prolonged progestin administration. In this study, we used canine cDNA microarrays to identify oncogenic determinants in progestin-induced canine hyperplasia (CMH) and spontaneous tumours (CMC) by comparing them to normal tissue. CMH profile indicated amplification of genes involved in cell proliferation, like PCNA, NPY and RAN, and also alterations in expression of transcription factors and cell adhesion molecules. In addition to amplified cell proliferation signature, CMC profile also identified major alterations in expressions of genes involved in cell motility, cytoskeletal organization and extracellular components. Overall, gene expression profile of CMH indicated an advantage for cell proliferation, whereas that of CMC indicated an advantage for proliferation as well as neoclassic transformation. In conclusion, our findings indicate strong oncogenic potential of progestins in canine mammary gland. In addition, CMC gene expression profile greatly contribute to the identification of their molecular abnormalities and may facilitate the identification of new therapeutic targets. Keywords: Expression profiling by using cDNA microarray
2009-02-09 | GSE13754 | GEO
Project description:Molecular mechanisms of the canine mammary malignancy. RNA isolated from canine mammary tumours of the 3rd grade of malignancy was pooled and hybrydized on the same microarray as RNA isolated from canine mammary tumours of the 1st grade of malignancy. Based on the results the "malignancy portrait" was assessed. Dye-swap experiment, gene expression in the most malignant canine mammary tumours (the 3rd grade of malignancy) was compared to the less malignant tumours (1st grade of malignancy).
2013-10-01 | E-GEOD-44033 | biostudies-arrayexpress
Project description:Using microarray technology, we aim to identify genes affecting the biology of mammary tumor cancers in dogs. Canine mammary gland tumors (CMGTs) are the most common neoplasms in sexually intact female dogs. CMGTs have been suggested as a model for studying human breast cancer because of several similarities, including the relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy.
2018-12-26 | GSE104733 | GEO