ABSTRACT: (ITS rRNA) - Ginger Beer produced using back-slopping fermentation: Characterization of volatile compounds, and physicochemical, microbiological, and sensory aspects
Project description:(16S rRNA) - Ginger Beer produced using back-slopping fermentation: Characterization of volatile compounds, and physicochemical, microbiological, and sensory aspects
Project description:We used two differentpretreatment methods to extract extracellular vesicles of ginger, one is juicing method and the other is enzyme digestion (cellulase + pectinase) method. After pretreatment, the extracellular vesicles of ginger were recovered by a series of gradient centrifugation and sucrose purification methods for proteomic detection, so as to analyze the differences in the types and contents of the extracellular vesicles of ginger proteins from the two methods.
Project description:Oomycetes, such as the broad host-range necrotrophic plant pathogen Pythium myriotylum, cause devastating crop losses. We have previously identified P. myriotylum as the major pathogen infecting ginger (Zingiber officinale) rhizomes in China with symptoms of Pythium soft rot (PSR) disease. Ginger is an important crop with global production estimated at approximately three million metric tonnes with about 20% of this production in China. To better understand how P. myriotylum infects ginger, transcriptomic analysis was performed on two P. myriotylum isolates (SWQ7 and SL2) infecting ginger leaves. From both of the isolates, there was a clear separation between the transcriptome replicates from the mycelial control condition and those from the infection of the ginger leaf. In SWQ7 and SL2, there were 2,110 and 2,513 genes upregulated during infection of ginger, respectively. Of the putative effectors, a subset of the NEP1-like toxin protein (NLP) effectors were highly induced during the infection of ginger leaves. Insights from the transcriptome highlight the important role of a subset of plant cell wall degrading enzymes (PCWDEs) and effectors in the pathogenicity of P. myriotylum towards ginger. The surprisingly large numbers of P. myriotylum PCWDEs and effectors within the genome may be due to the broad host-range of P. myriotylum whereby particular subsets of the PCWDEs and effectors are required for pathogenicity towards particular hosts.
Project description:We used two different pretreatment methods to extract extracellular vesicles of ginger, one is juicing method and the other is enzyme digestion (cellulase + pectinase) method. After pretreatment, the extracellular vesicles of ginger were recovered by a series of gradient centrifugation and sucrose purification methods for proteomic detection, so as to analyze the differences in the types and contents of the extracellular vesicles of ginger proteins from the two methods.
