Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Specification of a rostro-caudal axis in cortical assembloids through a polarized source of FGF8

ABSTRACT: Specification of a rostro-caudal axis in cortical assembloids through a polarized source of FGF8

PROVIDER: PRJNA963209 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14664005.fastq.gz	Fastqsanger.gz
	SRR14664006.fastq.gz	Fastqsanger.gz
	SRR14664007.fastq.gz	Fastqsanger.gz
	SRR14664008.fastq.gz	Fastqsanger.gz
	SRR14664009.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 50

Similar Datasets

Specification of a rostro-caudal axis in cortical assembloids through a polarized source of FGF8 (scRNA-Seq)

Project description:Current organoid protocols are widely used to mechanistically study cortical development, evolution and brain pathologies. While these systems generate all major cortical cell types in histologically distinct compartments, they lack cortical area topography, which is initiated by gradients of signaling molecules released by signaling organizers that establish transcriptionally distinct domains along the rostro-caudal axis of the developing cortex. Here, we engineer human cortical assembloids that combine an organizer-like structure expressing FGF8 with an elongated organoid design to enable the controlled modulation of FGF8 signaling along the longitudinal organoid axis. We demonstrate that individual polarized cortical assembloids (polCA) mount a position-dependent transcriptional program that matches the in vivo rostro-caudal gene expression patterns. Importantly, positional identity is lost in polCA carrying a mutation in the FGFR3 gene associated with temporal lobe malformations and intellectual disability in humans. Similar to in vivo, this program is accompanied by transcriptional divergence of radial glia cells and excitatory neurons and their segregation along the longitudinal axis of individual organoids. Thus, this method reproduces in vivo cortical topography in individual organoids and enables the analysis of area-related phenotypes underlying human disorders.

2024-05-03 | GSE231319 | GEO

Specification of a rostro-caudal axis in cortical assembloids through a polarized source of FGF8 (bulk RNA-Seq)

2024-05-03 | GSE231318 | GEO

Specification of a rostro-caudal axis in cortical assembloids through a polarized source of FGF8 (scRNA-Seq)

Project description:Specification of a rostro-caudal axis in cortical assembloids through a polarized source of FGF8 (scRNA-Seq)

| PRJNA963211 | ENA

Specification of a rostro-caudal axis in cortical assembloids through a polarized source of FGF8 (bulk RNA-Seq)

Project description:Specification of a rostro-caudal axis in cortical assembloids through a polarized source of FGF8 (bulk RNA-Seq)

| PRJNA963210 | ENA

Specification of a rostro-caudal axis in cortical assembloids through a polarized source of FGF8

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-05-03 | GSE231320 | GEO

Local source of FGF8 specifies a fronto-temporal axis in engineered human brain organoids (PolarOids)

Project description:The establishment of the fronto-temporal axis in the developing human cortex is achieved by temporary signaling activity coming from secondary organizers. However, studying this process remains challenging due to the limited access to fetal material and inaccuracy of animal models in mimicking human-specific aspects. Although human brain organoids provide a potential solution to study this, their lack of axial organization does not supply the appropriate positional information needed to recapitulate detailed patterning processes in situ. Here, we develop a method that combines a cell line expressing the morphogen FGF8 fused to organoids engineered with PDMS moulds representing the exact length of the developing brain, resulting in the formation of polarized cortical organoids, or PolarOids. Thanks to a dose-sensitive reporter for anterior cortical differentiation, we carefully titrate and time the fusion dynamics to elicit a gradient in the patterned tissue while preserving neural activity as assessed through electrophysiology. We show that our localized FGF8 source is sufficient to trigger a distance-dependent transcriptional signature that matches fronto-temporal cortical fates in vivo. Lastly, we use these PolarOids to model a disease-relevant FGFR3 mutation and describe the transcriptional loss of axial gradient formation underlying a dramatic cortical phenotype observed in patients. As such, Polaroids provide a powerful tool to model hitherto neglected human diseases caused by defects in antero-posterior patterning in the brain

2024-05-03 | GSE175592 | GEO

Single cell transcriptional profiling of mouse molar and incisor tooth assembloids

Project description:Throughout the various stages of tooth development, reciprocal epithelial-mesenchymal interactions are the driving force, for instance crucially involved in the differentiation of mature enamel-forming ameloblasts and dentin-producing odontoblasts. Here we established mouse tooth ‘assembloids’, comprised of tooth organoid-derived dental epithelial cells (from mouse molars and incisors) cultured together with molar dental pulp stem cells (DPSCs), to mimic these developmental interactions. Assembloids from both tooth types were grown both in basal- and differentiation-inducing conditions. Single cell transcriptomics analysis was applied to in detail characterize and validate the newly developed mouse tooth assembloid model and evaluate the induced differentiation processes.

2023-04-19 | E-MTAB-12544 | biostudies-arrayexpress

Single cell RNA sequencing (scRNAseq) of stromal cells from mouse colon assembloids and epithelial cells from colon assembloids, organoids, and tissue

Project description:We established a colon assembloid system comprising epithelium and diverse subtypes of stromal cells. To explore how closely assembloids resemble the native tissue, we performed the transcriptional profiling of stromal cells from assembloids and epithelial cells from assembloids, organoids, and colon tissue at the single-cell level.

2023-05-12 | GSE231716 | GEO

Single cell RNA seq of mouse bladder and bladder assembloids

Project description:To investigate whether the bladder assembloids recapitulate cell compositions, in addition to the gene expression patterns of each cell type in the adult bladder, we performed single-cell RNA-seq of bladder assembloids and mouse adult bladders, and compared the transcriptional profiles between them at the single-cell level.

2020-09-27 | GSE141348 | GEO

Effect of FGF8 on bovine granulosa cells

Project description:FGF8 induction of immediate-early response genes

2013-10-10 | GSE41489 | GEO